Preparation method and application of pulmonary artery tissue unicellular suspension

Abstract

The invention provides a preparation method of a pulmonary artery tissue unicellular suspension for unicellular sequencing. The unicellular suspension can be obtained by separating, cleaning, trimming, incubating and enzymatically digesting pulmonary endarterectomy tissues. According to the method disclosed by the invention, while the cell quality is ensured, a large number of separated cells derived from the pulmonary endarterectomy tissues can be obtained at a time; and a used reagent is simple and convenient to operate, and the method has good practical application value.

Description

technical field [0001] The invention provides a method for preparing a single-cell suspension of pulmonary artery endarterectomy tissue used for single-cell sequencing, which belongs to the field of biomedical technology, and specifically relates to a method for preparing a single-cell suspension of pulmonary artery endarterectomy tissue for single-cell sequencing Preparation method and application. Background technique [0002] Chronic thromboembolic pulmonary hypertension (CTEPH) is a disease characterized by dyspnea, fatigue, and reduced exercise tolerance due to unresolved thrombus emboli occluding the proximal pulmonary artery with accompanying distal pulmonary vascular Remodeling, leading to increased pulmonary vascular resistance, progressive increase in pulmonary artery pressure and progressive increase in right heart load, eventually progressing to right heart failure, is a serious, progressive, and fatal disease. The incidence of CTEPH is increasing year by year. ...

AI Technical Summary

Problems solved by technology

The current implementation method is to send the single-cell separation equipment to the hospital, and the fresh tissue obtained by the operation is immediately digested and subsequently separated, which greatly limits the cost, time, and place of the experiment.

Moreover, single-cell sequencing experiments require a large number of isolated cells with cell viability higher than 90% (primary cell viability higher than 70%). Subculture cells are not suitable for single-cell sequencing

It is not easy to obtain a high-viability single-cell suspension from stripped pulmonary artery tissue, and the single-cell suspension is also difficult to transport, because the cell viability cannot be guaranteed during transportation

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