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Preparation method and application of ganoderma lucidum mycelium polysaccharide

A technology of ganoderma lucidum mycelium and polysaccharides, applied in the fields of application, polysaccharide/gum-containing food ingredients, medical preparations containing active ingredients, etc., can solve the problems of low purity of ganoderma lucidum polysaccharides, high extraction cost, waste of raw materials, etc., and achieve comprehensive Good utilization effect, simplified process steps, and improved extraction rate

Inactive Publication Date: 2020-09-04
广州颜如玉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this extraction method can indeed obtain a certain amount of Ganoderma lucidum polysaccharides, the purity of Ganoderma lucidum polysaccharides is not high, and it uses Ganoderma lucidum as the raw material for extraction, which causes a lot of waste of raw materials in the extraction process, and the extraction cost is high, so it is not suitable for industrial production.
[0006] In summary, there are generally technical problems such as low purity of Ganoderma lucidum polysaccharides, serious waste of raw materials, and unsuitability for industrial production in the prior art.

Method used

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  • Preparation method and application of ganoderma lucidum mycelium polysaccharide

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Embodiment 1

[0029] Embodiment 1, a kind of Ganoderma lucidum mycelia polysaccharide

[0030] The preparation method of described Ganoderma lucidum mycelia polysaccharide, comprises the steps:

[0031] S1, get the dried Ganoderma lucidum mycelium and put it into a pulverizer and pulverize to 8 μm, then add 8 times the amount of organic solvent and carry out degreasing treatment, the organic solvent is 2 by mass ratio by propylene glycol methyl ether, acetone and dimethyl sulfoxide: 4:1 composition, the degreasing treatment time is 1h, centrifuged, the lower layer is removed and dried at 50°C to obtain coarse powder of Ganoderma lucidum mycelium;

[0032] S2, get the Ganoderma lucidum mycelium coarse powder that step S1 makes, add the deionized water of 10 times amount, then carry out supercritical CO 2 Extraction, supercritical CO 2 The extraction conditions are: pressure 30Mpa, extraction temperature 50°C, extraction time 4h; filter to obtain water extract;

[0033] S3, take the water ...

Embodiment 2

[0036] Embodiment 2, a kind of Ganoderma lucidum mycelia polysaccharide

[0037] The preparation method of described Ganoderma lucidum mycelia polysaccharide, comprises the steps:

[0038] S1, take the dried Ganoderma lucidum mycelium and put it into a pulverizer to pulverize to 8.5 μm, then add 8.5 times the amount of organic solvent for degreasing treatment, the organic solvent is composed of propylene glycol methyl ether, acetone and dimethyl sulfoxide in a mass ratio of 3 : 5:2 composition, the degreasing treatment time is 1.5h, centrifuged, the lower layer of sediment is taken and dried at 55°C to obtain coarse powder of Ganoderma lucidum mycelium;

[0039] S2, get the Ganoderma lucidum mycelium coarse powder that step S1 makes, add 14 times of deionized water, then carry out supercritical CO 2 Extraction, supercritical CO 2 The extraction conditions are: pressure 31Mpa, extraction temperature 53°C, extraction time 4.2h; filter to obtain water extract;

[0040] S3, tak...

Embodiment 3

[0043] Embodiment 3, a kind of Ganoderma lucidum mycelia polysaccharide

[0044] The preparation method of described Ganoderma lucidum mycelia polysaccharide, comprises the steps:

[0045] S1, get the dried ganoderma lucidum mycelium and put it into a pulverizer and pulverize to 9 μm, then add 9 times the amount of organic solvent and carry out degreasing treatment, the organic solvent is 2 by mass ratio by propylene glycol methyl ether, acetone and dimethyl sulfoxide: 6:1 composition, the degreasing treatment time is 2 hours, centrifuged, the lower layer is removed and dried at 58°C to obtain coarse powder of Ganoderma lucidum mycelium;

[0046] S2, get the Ganoderma lucidum mycelium coarse powder that step S1 makes, add 16 times of deionized water, then carry out supercritical CO 2 Extraction, supercritical CO 2 The extraction conditions are: pressure 33Mpa, extraction temperature 55°C, extraction time 4.5h; filter to obtain water extract;

[0047] S3, take the water extr...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to ganoderma lucidum mycelium polysaccharide as well as a preparation method and application thereof. Ganoderma lucidum mycelia rich in ganoderma lucidum polysaccharides are adopted as an extraction raw material, and the ganoderma lucidum mycelia polysaccharides are prepared through the process steps of rawmaterial pretreatment, degreasing treatment, supercritical CO2 extraction, enzymolysis, alcohol precipitation and the like. The extraction rate of the ganoderma lucidum mycelium polysaccharides prepared by the preparation method of the ganoderma lucidum mycelium polysaccharides reaches 15.4% or above, and the purity of the ganoderma lucidum mycelium polysaccharides reaches 76.1% or above. The ganoderma lucidum mycelium polysaccharide is extracted by adopting a supercritical CO2 extraction technology and an enzymolysis technology, so that the extraction rate of the polysaccharide is increased,the waste of raw materials is avoided, the production cost is saved, and the prepared ganoderma lucidum mycelium polysaccharide is applied to preparation of health care products and medicines, has good effects, and is remarkable in economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a ganoderma lucidum mycelia polysaccharide and a preparation method and application thereof. Background technique [0002] Ganoderma lucidum mycelium is the mycelium formed by the germination of Ganoderma lucidum spores, and the constituent substances required by the fruiting body of Ganoderma lucidum. More than half of the nutrients come from the nutrients stored in the weight of the mycelium. The content of Ganoderma lucidum is generally many times higher than that of Ganoderma lucidum fruiting body and Ganoderma lucidum spore powder. [0003] Ganoderma lucidum mycelium polysaccharide is composed of three strands of monosaccharide chains, and its configuration is similar to DNA and RNA. It is a helical three-dimensional configuration, and the helical layers are mainly fixed by hydrogen bonds. The polysaccharides of Ganoderma lucidum mycelium have significa...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P35/00A61P39/06A61P9/00A61P9/12A61P11/14A61P37/08A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P9/00A61P9/12A61P11/14A61P35/00A61P37/02A61P37/08A61P39/06C08B37/0003A23V2200/302A23V2200/304A23V2200/314A23V2200/308A23V2200/324A23V2200/326A23V2250/51Y02P20/54
Inventor 王中振谢涛
Owner 广州颜如玉生物科技有限公司
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