Method for obtaining regenerated plants by tissue culture of flowers of Amorphophallus bulbifer (Roxb.) Blume

A technology for regenerating plants and flower organs, applied in the biological field, can solve problems such as the lack of healthy taro seeds, and achieve the effects of convenient operation, high multiplication efficiency, and low energy consumption

Active Publication Date: 2020-09-01
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method for obtaining regenerated plants through tissue culture of A. bulbils flower pots in view of the deficiencies in the prior art. The method of tissue culture and rapid propagation of A. bulbils A. konjac flower pots can be used to breed healthy seedlings (planting taro) on a large scale and efficiently. , to solve the serious shortage of healthy taro in the development of bulbil konjac industry, which has practical significance for promoting the development of konjac industry, and has reference value for opening up a new way of research on detoxification technology for konjac seedlings

Method used

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  • Method for obtaining regenerated plants by tissue culture of flowers of Amorphophallus bulbifer (Roxb.) Blume
  • Method for obtaining regenerated plants by tissue culture of flowers of Amorphophallus bulbifer (Roxb.) Blume
  • Method for obtaining regenerated plants by tissue culture of flowers of Amorphophallus bulbifer (Roxb.) Blume

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Disinfection of explants: Take the unexpanded inflorescence of Amorphophallus konjac spathe, remove the outer flower bud scales, wash the surface of the inflorescence with saturated washing powder solution, rinse with tap water 3 times, and use 75 After wiping the entire inflorescence surface 3 times evenly with % alcohol, cut the spathe and take the male bud.

[0027] 2. Callus induction: take the male buds and inoculate them into the callus induction medium, and culture them in the dark at 26°C to obtain the callus (see figure 1 A), the callus induction rate is shown in Table 1.

[0028] Callus induction medium:

[0029] Modified MS+NH 4 Cl 1100mg / L+KNO 3 2500mg / L+Ca(NO 3 ) 2 615mg / L+NaH 2 PO 4 160mg / L+MgSO 4 ·7H 2 O 370mg / L+inositol 100mg / L+niacin 0.8mg / L+pyridoxine hydrochloride 0.1mg / L+thiamine hydrochloride 5mg / L+aminoacetic acid 2.0mg / L+2,4-D1mg / L+6-BA3 mg / L+ agar 6000mg / L+ sucrose 30000mg / L+ calcium pantothenate 5mg / L+ biotin 0.05mg / L, adjust th...

Embodiment 2

[0039] 1. Disinfection of explants: Take the unexpanded inflorescence of Amorphophallus konjac spathe, remove the outer flower bud scales, wash the surface of the inflorescence with saturated washing powder solution, rinse with tap water 3 times, and use 75 After wiping the entire inflorescence surface 3 times evenly with % alcohol, cut the spathe and take the male bud.

[0040] 2. Callus induction: the male buds were inoculated into the callus induction medium, and cultured in the dark at 25°C to obtain the callus. The callus induction rate is shown in Table 1.

[0041] Callus induction medium:

[0042] Modified MS+NH 4 Cl 1000mg / L+KNO 3 2600mg / L+Ca(NO 3 ) 2 625mg / L+NaH 2 PO 4 165mg / L+MgSO 4 ·7H 2 O 360mg / L+inositol 120mg / L+niacin 0.5mg / L+pyridoxine hydrochloride 2mg / L+thiamine hydrochloride 2.5mg / L+aminoacetic acid 1.0mg / L+2,4-D 3mg / L+6-BA 2mg / L+ agar 5500mg / L+ sucrose 28000mg / L+ calcium pantothenate 3mg / L+ biotin 0.1mg / L, adjust the pH of the medium to 6.0 befo...

Embodiment 3

[0052] 1. Disinfection of explants: Take the unexpanded inflorescence of Amorphophallus konjac spathe, remove the outer flower bud scales, wash the surface of the inflorescence with saturated washing powder solution, rinse with tap water 3 times, and use 75 After wiping the entire inflorescence surface 3 times evenly with % alcohol, cut the spathe and take the male bud.

[0053] 2. Callus induction: male buds were inoculated into callus induction medium, and cultured in the dark at 28°C to obtain callus. The callus induction rate is shown in Table 1.

[0054] Callus induction medium:

[0055] Modified MS+NH 4 Cl 1000mg / L+KNO 3 2600mg / L+Ca(NO 3 ) 2 615mg / L+NaH 2 PO 4 165mg / L+MgSO 4 ·7H 2 O 375mg / L+inositol 105mg / L+niacin 0.2mg / L+pyridoxine hydrochloride 3mg / L+thiamine hydrochloride 0.5mg / L+aminoacetic acid 2.0mg / L+2,4-D 10mg / L+6-BA 1mg / L+ agar 6300mg / L+ sucrose 33000mg / L+ calcium pantothenate 1mg / L+ biotin 0.2mg / L, adjust the pH of the medium to 6.2 before steriliz...

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Abstract

The invention belongs to the technical field of biology, and relates to a method for obtaining regenerated plants by tissue culture of flowers of Amorphophallus bulbifer (Roxb.) Blume. The method comprises the processes of explant disinfection, callus induction, corm like body induction and proliferation, corm like body germination, rooting induction and the like. The method is simple in process,convenient to operate and relatively low in energy consumption; the flowers of Amorphophallus bulbifer (Roxb.) Blume are used as explant materials for induction to obtain callus, and then corm like bodies are obtained by induction and then are proliferated, so that the proliferation efficiency is relatively high; the corm like bodies are cultured into complete plants, and the obtained clone plantsare derived from clean organs without germs or viruses; healthy seedlings (seeds) of Amorphophallus bulbifer (Roxb.) Blume can be efficiently bred in a large scale; the problem of serious shortage ofthe healthy seeds of Amorphophallus bulbifer (Roxb.) Blume in development of the industry of Amorphophallus bulbifer (Roxb.) Blume is solved; and the method has practical significance for promoting development of the industry of Amorphophallus bulbifer (Roxb.) Blume, and has reference value for developing a new way for researching a detoxification technology for seedlings of Amorphophallus bulbifer (Roxb.) Blume.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for obtaining regenerated plants by plant tissue culture, in particular to a method for obtaining regenerated plants by tissue culture of bulbil bud konjac flower organ. Background technique [0002] Konjac is a perennial monocotyledonous herb belonging to Araceae Konjac, with well-developed underground bulbs. It is the only higher plant found in the plant kingdom that can synthesize glucomannan in large quantities. Its enlarged underground bulbs are rich in glucomannan, starch, Protein, etc. Konjac flour processed by it has good gelling properties, water holding capacity, compounding properties, high viscosity and stability, etc., and is widely used in food, health care, medicine, construction, daily chemicals, oil drilling, etc. field, with extremely high development and utilization value. Konjac has a history of more than 2,000 years of cultivation in my country. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 潘登浪李炜芳曾宪海林位夫邹积鑫郑定华曾精李哲
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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