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Method for detecting fertility of sheep

A fertility and detection line technology, applied in the field of detection, can solve problems such as economic losses, and achieve the effects of reducing economic losses, improving production efficiency, and improving reproduction rate

Active Publication Date: 2020-08-28
昆明天沃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the prior art are: a ewe needs 10--12 months from birth to the first hair stage, and the sexual maturity time of the ram is greater than 6 months. When the ram or ewe reaches the mating age and cannot breed normally, will cause economic loss

Method used

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  • Method for detecting fertility of sheep
  • Method for detecting fertility of sheep

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Follicle-stimulating hormone FSH test paper is prepared by the following method:

[0041] 1) Prepare the components as follows:

[0042] 11) Gold solution: Add 2ml of chloroauric acid solution with a mass concentration of 1%, and sodium citrate with a mass concentration of 1% to 100ml of purified water, heat until the solution turns purple, and cool to room temperature to obtain a gold solution;

[0043] 12) Blocking solution: Dissolve 0.005g bovine serum albumin in 300ul purified water, add 30ul potassium carbonate solution, and mix well to obtain 330ul blocking solution;

[0044] 13) Sample pad treatment solution: completely dissolve 0.605g of tricarboxymethylaminomethane and 1.5g of sodium chloride in 100ml of purified water to obtain a sample pad treatment solution;

[0045] 14) Colloidal gold conjugate diluent: mix 0.605g tricarboxymethylaminomethane, 0.2g polyvinylpyrrolidone, 5g sucrose, 0.9g sodium chloride, 0.5g bovine serum albumin, 3ml sheep serum, 0.15ml Tw...

Embodiment 2

[0063] The method for detecting sheep fertility comprises the following steps:

[0064] 1) Collect 1ml of ewe blood from the jugular vein of ewes by using blood collection needles and anticoagulant blood vessels, let it stand in a cool place for 1 hour, and separate the serum;

[0065] 2) Insert the follicle-stimulating hormone FSH detection test paper of Example 1 into the serum of step 1) for 5 seconds, take it out, and observe whether the quality control line C and the detection line T in the test paper are colored after 15 minutes:

[0066] 3) The color development results are as follows:

[0067] The quality control line C of the test paper has no color, and the test line T has color, indicating that the test result is invalid;

[0068] Immediately repeat steps 1) and 2) to observe whether the quality control line C and the detection line T in the test paper develop color, and the color development results are as follows:

[0069] Both the quality control line C and the...

Embodiment 3

[0072] The method for detecting sheep fertility comprises the following steps:

[0073] 1) Collect 10 ml of sheep blood from the ewe's jugular vein with a blood collection needle and anticoagulant blood vessel, let it stand in a cool place for 2 hours, and separate the serum;

[0074] 2) Insert the follicle-stimulating hormone FSH detection test paper of Example 1 into the serum of step 1) for 10 seconds, take it out, and observe whether the quality control line C and the detection line T in the test paper are colored after 18 minutes:

[0075] 3) The color development results are as follows:

[0076] The quality control line C on the test paper develops color, but the test line T does not develop color, which only indicates that the FSH level of the sheep is normal. Repeat steps 1) and 2) for detection two days later, and the results are as follows:

[0077] Both the quality control line C and the detection line T of the test paper are colored, and the following comparisons ...

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Abstract

The invention provides a method for detecting fertility of sheep. The method comprises the following steps of: 1) collecting sheep blood or urine; 2) inserting follicle stimulating hormone FSH detection test paper of sheep into serum or urine for 5-10 seconds, taking out the detection test paper, and observing whether a quality control line C and a detection line T in the detection test paper develop colors or not after 15-18 minutes; 3) indicating that a detection result is invalid if the test paper control line C does not develop colors and the detection line T develops colors; if the test paper control line C develops color and the detection line T does not develop color, indicating that the follicle stimulating hormone FSH level of the sheep is normal; if the test paper control line Cand the detection line T develop colors, and comparing the developed colors with an FSH colorimetric card; if FSH is less than 5mIU / ml, indicating that the fertility of the sheep is normal; if the FSHis 5-10mIU / ml, indicating that the fertility of the sheep declines; and if the FSH is greater than 10mIU / ml, indicating that the sheep have no fertility. The method can detect whether the sheep havefertility or not conveniently, simply, quickly and accurately

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detection of sheep fertility, and belongs to the technical field of animal detection methods. Background technique [0002] At present, the empty pregnancy rate of sheep is 6%-7%, and its fertility is mainly affected by factors such as genetic selection, age, nutritional status and environment of ewes. The evaluation of sheep fertility is usually expressed by the reproductive efficiency of the whole flock of sheep, or the number of lambs weaned per adult ewe per year. In order to improve the fertility, most of the offspring of multi-fetal sheep will be selected as breeding ewes. Select the offspring of high producing ewes. The disadvantages of the prior art are: a ewe needs 10--12 months from birth to the first hair stage, and the sexual maturity time of the ram is greater than 6 months. When the ram or ewe reaches the mating age and cannot breed normally, will cause economic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/558G01N33/532G01N33/543
CPCG01N33/76G01N33/558G01N33/532G01N33/54313G01N2333/59Y02A40/70
Inventor 金义达
Owner 昆明天沃生物科技有限公司
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