Method for detecting fertility of cows

A fertility and detection line technology, applied in the field of detection, can solve problems such as economic losses, achieve the effects of reducing economic losses, improving production efficiency, and promoting intensive management

Active Publication Date: 2020-08-28
昆明天沃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drawbacks of the prior art are: a cow needs 16--22 months from birth to the first estrus period, and a bull needs more than 24--36 months for sexual maturity. Breeding will bring economic loss

Method used

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  • Method for detecting fertility of cows
  • Method for detecting fertility of cows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The bovine follicle-stimulating hormone FSH test paper is prepared by the following method:

[0041] 1) Prepare the components as follows:

[0042] 11) Gold solution: Add 2ml of chloroauric acid solution with a mass concentration of 1%, and sodium citrate with a mass concentration of 1% to 100ml of purified water, heat until the solution turns purple, and cool to room temperature to obtain a gold solution;

[0043] 12) Blocking solution: Dissolve 0.005g bovine serum albumin in 300ul purified water, add 30ul potassium carbonate solution, and mix well to obtain 330ul blocking solution;

[0044] 13) Sample pad treatment solution: completely dissolve 0.605g of tricarboxymethylaminomethane and 1.5g of sodium chloride in 100ml of purified water to obtain a sample pad treatment solution;

[0045] 14) Colloidal gold conjugate diluent: mix 0.605g tricarboxymethylaminomethane, 0.2g polyvinylpyrrolidone, 5g sucrose, 0.9g sodium chloride, 0.5g bovine serum albumin, 3ml sheep serum...

Embodiment 2

[0063] A method for detecting cattle fertility, comprising the following steps:

[0064] 1) Collect 8ml of morning urine from cows;

[0065] 2) Insert the follicle-stimulating hormone FSH detection test paper of Example 1 into the urine of step 1) for 6 seconds, take it out, and observe whether the quality control line C and detection line T in the test paper are colored after 16 minutes:

[0066] 3) The color development results are as follows:

[0067] The quality control line C of the test paper has no color, and the test line T has color, indicating that the test result is invalid;

[0068] Repeat steps 1) and 2) the next day, and observe whether the quality control line C and the detection line T in the test paper develop color, and the color development results are as follows:

[0069] Both the quality control line C and the detection line T of the test paper are colored, and the following comparisons are made between the color developed and the FSH color card;

[007...

Embodiment 3

[0072] A method for detecting cattle fertility, comprising the following steps:

[0073] 1) Collect 7ml of urine that the cow urinates 1-2 hours after the last urination;

[0074] 2) Insert the follicle-stimulating hormone FSH detection test paper of Example 1 into the serum of step 1) for 9 seconds, take it out, and observe whether the quality control line C and detection line T in the test paper develop color after 17 minutes:

[0075] 3) The color development results are as follows:

[0076] The quality control line C on the test paper develops color, but the test line T does not develop color, indicating that the FSH level of the cow is low. Repeat steps 1) and 2) on the third day for detection, and the results show that: the test paper quality control line C and The detection line T is all colored, use this color and HCG color card as figure 2 As shown, carry out following comparison; Test paper quality control line C and detection line T all develop color, carry out f...

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PUM

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Abstract

The invention provides a method for detecting the fertility of cows. The method comprises the following steps of: collecting morning urine or urine urinated 1-2 hours after last urination of cows; inserting bovine follicle stimulating hormone FSH detection test paper into the urine obtained in the step (1) for 5-10 seconds, and indicating that a detection result is invalid after observing that a test paper quality control line C does not develop color and a detection line T develops color after 15-18 minutes; indicating that a follicle-stimulating hormone FSH level of the cows is normal if thequality control line C develops color and the detection line T does not develop color; comparing developed colors with an FSH colorimetric card if both the quality control line C and the detection line T develop colors; indicating that the fertility of the cows is normal if the FSH is less than 5mIU / ml; indicating that the fertility of the cows is reduced if the FSH is 5-10 mIU / ml; and indicatingthat the cows have no fertility if the FSH is larger than 10 mIU / ml. The method overcomes the defect that the fertility of the cows cannot be directly detected and more mating opportunities are lostin the prior art, the reproductive rate is effectively increased, economic losses are reduced, and the production benefits of the cows are increased.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detection of bovine fertility, and belongs to the technical field of animal detection methods. Background technique [0002] At present, the fertility of cattle is usually evaluated by indicators such as empty pregnancy period, percentage of mating, insemination rate, abortion rate, and calf mortality rate after the cow has grown into adulthood. The drawbacks of the prior art are: a cow needs 16--22 months from birth to the first estrus period, and a bull needs more than 24--36 months for sexual maturity. Breeding will bring economic losses. In the embryonic plasma of primates and other mammals, the follicle-stimulating hormone content will reach a peak in the mid-embryo stage, and will gradually decrease in the late stage of the embryo, and continue until the production stage. The reproductive organs of the newborn calf are in a quiescent state, and the hypothalamic gonadotro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/558G01N33/543G01N33/532
CPCG01N33/76G01N33/558G01N33/532G01N33/54313G01N2333/59
Inventor 金义达
Owner 昆明天沃生物科技有限公司
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