Method for detecting fertility of cows
A fertility and detection line technology, applied in the field of detection, can solve problems such as economic losses, achieve the effects of reducing economic losses, improving production efficiency, and promoting intensive management
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Embodiment 1
[0040] The bovine follicle-stimulating hormone FSH test paper is prepared by the following method:
[0041] 1) Prepare the components as follows:
[0042] 11) Gold solution: Add 2ml of chloroauric acid solution with a mass concentration of 1%, and sodium citrate with a mass concentration of 1% to 100ml of purified water, heat until the solution turns purple, and cool to room temperature to obtain a gold solution;
[0043] 12) Blocking solution: Dissolve 0.005g bovine serum albumin in 300ul purified water, add 30ul potassium carbonate solution, and mix well to obtain 330ul blocking solution;
[0044] 13) Sample pad treatment solution: completely dissolve 0.605g of tricarboxymethylaminomethane and 1.5g of sodium chloride in 100ml of purified water to obtain a sample pad treatment solution;
[0045] 14) Colloidal gold conjugate diluent: mix 0.605g tricarboxymethylaminomethane, 0.2g polyvinylpyrrolidone, 5g sucrose, 0.9g sodium chloride, 0.5g bovine serum albumin, 3ml sheep serum...
Embodiment 2
[0063] A method for detecting cattle fertility, comprising the following steps:
[0064] 1) Collect 8ml of morning urine from cows;
[0065] 2) Insert the follicle-stimulating hormone FSH detection test paper of Example 1 into the urine of step 1) for 6 seconds, take it out, and observe whether the quality control line C and detection line T in the test paper are colored after 16 minutes:
[0066] 3) The color development results are as follows:
[0067] The quality control line C of the test paper has no color, and the test line T has color, indicating that the test result is invalid;
[0068] Repeat steps 1) and 2) the next day, and observe whether the quality control line C and the detection line T in the test paper develop color, and the color development results are as follows:
[0069] Both the quality control line C and the detection line T of the test paper are colored, and the following comparisons are made between the color developed and the FSH color card;
[007...
Embodiment 3
[0072] A method for detecting cattle fertility, comprising the following steps:
[0073] 1) Collect 7ml of urine that the cow urinates 1-2 hours after the last urination;
[0074] 2) Insert the follicle-stimulating hormone FSH detection test paper of Example 1 into the serum of step 1) for 9 seconds, take it out, and observe whether the quality control line C and detection line T in the test paper develop color after 17 minutes:
[0075] 3) The color development results are as follows:
[0076] The quality control line C on the test paper develops color, but the test line T does not develop color, indicating that the FSH level of the cow is low. Repeat steps 1) and 2) on the third day for detection, and the results show that: the test paper quality control line C and The detection line T is all colored, use this color and HCG color card as figure 2 As shown, carry out following comparison; Test paper quality control line C and detection line T all develop color, carry out f...
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