A special culture medium and culture method for scaffold-free organoids of breast cancer
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A culture medium and organoid technology, which is applied in the special medium and culture field of breast cancer organoids, can solve the problems of affecting the cell state, cumbersome removal of scaffold materials, poor cell growth, etc., achieving a high success rate and maintaining tumor specificity. , the effect of high specificity of drug susceptibility results
Active Publication Date: 2021-11-16
浙江弘瑞医疗科技有限公司
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[0006] In view of the existing organoid culture, it is necessary to add scaffold materials such as Matrigel and hydrogel to the culture medium, resulting in poor cell growth, cumbersome removal of scaffold materials in the later stage, and affecting the state of cells. The present invention provides a A new special medium suitable for the establishment of breast cancer organoids, which can be used without being limited to scaffold materials, and can also obtain good culture results
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Embodiment 1
[0060] Example 1 Culture of Breast Cancer 3D Organoids
[0061] 1. Special medium formula for breast cancer organoids
[0062] The ingredients, the concentration of each ingredient or the volume percentage content are shown in Table 1.
[0063] Table 1 Composition of special medium for breast cancer organoids
[0064]
[0065]
[0066] 2. Patient Breast Cancer Tissue Processing
[0067] Tumor tissues were mainly derived from surgically resected specimens and biopsies obtained from breast cancer patients, and patients were required not to have received any antitumor therapy. In order to ensure the activity of isolated tumor primary cells, the blood clots, necrotic tissue, fat and connective tissue on the surgically resected tumor specimens were immediately cut off, and then treated with double antibodies (500 U / mL penicillin and 500 μg / mL streptavidin) prime) PBS solution, and then cut the tissue pieces to 1mm with tissue scissors 3 left and right size. The tissue ca...
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Abstract
The invention discloses a scaffold-free tumor organoid culture medium and a culture method for breast cancer, including N-2, B27, Noggin, penicillin / streptomycin double antibody, EGF, FGF10, Y-27632, A83-01, SB202190, N‑Acetylcysteine, R‑Spondin, Nicotinamide, Wnt3A, KGF, Gentamicin, Heregulin, Fetal Bovine Serum, and DMEM‑F / 12. The culture medium and culture method can help to establish a breast cancer tumor organoid that does not require scaffold materials and can maintain a high degree of tumor specificity, realizes controllable size, regular spherical shape, high success rate, short time, and specific drug sensitivity results high. The culture medium and the culture method of the invention can provide an ideal model for the occurrence and development of mammary gland tumors, in vitro drug sensitivity screening and drug resistance mechanism research.
Description
technical field [0001] The invention relates to the technical field of cell culture, in particular to a special culture medium and a culture method for breast cancer organoids. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, and its incidence is increasing year by year. In September 2018, the International Agency for Research on Cancer (IARC), a subsidiary of the WHO, released the "2018 Global Cancer Statistics", and the incidence and mortality of breast cancer both won the first place, making it the number one cancer killer of women in the world. There are 210,000 new cases of breast cancer patients in my country every year, and the growth rate of the incidence rate is twice the global average growth rate, ranking first in the world. In my country, the incidence rate of breast cancer in cities is the second among female malignant tumors. In some big cities, the incidence rate of breast cancer has risen to the first place, an...
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