Treatment method for improving tissue culture germination of rhodomyrtus tomentosa seeds and reducing pollution rate

Abstract

The invention relates to a treatment method for improving tissue culture germination of rhodomyrtus tomentosa seeds and reducing the pollution rate. The method comprises the following steps of (1) performing seed pretreatment: disinfecting the peeled rhodomyrtus tomentosa seeds by using a sterilization solution, washing the seeds with water, and drying the seeds; (2) performing seed soaking: sequentially soaking the seeds pretreated in the step (1) with a CaCl2 aqueous solution and a gibberellin aqueous solution, cleaning the seeds with water, and drying the seeds; (3) performing wet sand treatment: mixing the soaked seeds obtained in the step (2) with wet river sand, and performing stacking at 0-8 DEG C; (4) performing sterile treatment on the seeds: cleaning the seeds subjected to wet sand treatment in the step (3) with water, sequentially disinfecting the seeds with an ethanol aqueous solution, a mercuric chloride aqueous solution and a sodium hypochlorite aqueous solution, and cleaning the seeds with water after each disinfection; and (5) performing germination culture: finally drying the sterilely treated seeds obtained in the step (4), and culturing the seeds in a culture medium. The method provided by the invention can effectively improve the germination rate of the rhodomyrtus tomentosa seeds and reduce the pollution rate of seed tissue culture.

Description

technical field [0001] The invention relates to the technical field of modern Chinese herbal medicine cultivation, in particular to a treatment method for improving the tissue culture germination of myrtle seeds and reducing the pollution rate. Background technique [0002] Myrtle (Rhodomyrtus tomentosa (Ait.) Hask) is an evergreen shrub of the family Myrtaceae. It is also known as Gangren, Shanren, Shandanzai, Taoniang, etc. It is native to southeastern, southern, and Southwest and tropical and subtropical Asia. Myrtle combines ornamental, medicinal, nutritional and health care functions. It has dark green leaves, bright colors, and edible fruits. It has high ornamental value and edible and medicinal value. It is an excellent wild plant resource. It has made great contributions to the greening of barren hills and soil and water conservation in South China, and is also an important nectar source, medicinal and spice tree species. [0003] Myrtle is a traditional Chinese me...

AI Technical Summary

Problems solved by technology

However, the seeds selected are newly ripe myrtle seeds stripped from mature fresh fruits in December. Although the germination rate of seeds in this period is much higher than that of seeds stored for a period of time, the time limit for seed germination must be close to that of seed collection. The collection time is not conducive to the implementation of large-scale industrialization

However, during the storage period, myrtle seeds will greatly reduce their seed vigor and germination rate as the storage time prolongs, and the germination time will prolong.

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