Synthesis method of L-amino acid

A synthesis method, amino acid technology, applied in the direction of biochemical equipment and methods, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., can solve the problem of single amino acid composition, inability to obtain, and reduce simultaneous intake A variety of nutrients and other issues, to achieve the effect of improving drying quality and improving synthesis quality

Inactive Publication Date: 2020-08-11
沈红超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to make up for the deficiencies of the prior art, solve the problem that in the prior art, L-amino acid is only used as a basic nutrient component of an organism, and the amino acid component is single, and the organism cannot obtain more nutrients that it wants to ingest when ingested. Unable to meet the needs of multiple nutritional components of organisms at the same time, reducing the opportunity for organisms to take in multiple nutritional components at the same time, a method for synthesizing L-amino acids proposed by the present invention

Method used

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  • Synthesis method of L-amino acid
  • Synthesis method of L-amino acid
  • Synthesis method of L-amino acid

Examples

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preparation example Construction

[0030] like Figure 1 to Figure 6 Shown, a kind of synthetic method of L-amino acid of the present invention, this synthetic method comprises the following steps:

[0031] S1. Weigh a certain amount of soybean meal, add distilled water at a solid-to-liquid ratio of 1:25, incubate in a water bath at 50°C for 1 hour, adjust the pH to 8.5, add 10% trypsin, hydrolyze for 4 hours, add 8% papain, and hydrolyze 4h, inactivate the enzyme at 90°C for 10min, cool and stand still, centrifuge to separate the supernatant, and obtain the L-amino acid enzymatic hydrolysis solution;

[0032] S2. According to the molar mass ratio of L-amino acid and selenium salt as 2:1, add sodium selenite to the amino acid enzymatic hydrolysis solution, stir evenly, adjust the pH to 9.0, react at 80°C for 2 hours, and cool;

[0033] S3. Centrifuge to remove impurities, measure the content of free amino nitrogen, concentrate the obtained solution to 1 / 5 of the original volume, add 5 times the volume of 95% e...

Embodiment approach

[0038] As an embodiment of the present invention, inclined surfaces are provided on both sides of the installation ring 11; during operation, during the reciprocating lateral movement of the installation ring 11, the selenized composite L- When the amino acid is scraped, the scraped selenized compound L-amino acid will be squeezed, which will hinder the installation ring 11. By setting up the slope, the scraped amino acid can be rolled and pushed to avoid the selenized compound L- The stacking extrusion between the amino acids reduces the resistance to the reciprocating and lateral movement of the installation ring 11 , so that the installation ring 11 can move normally.

[0039] As an embodiment of the present invention, a support spring 18 is fixedly connected to the inner wall of the stirring rod 12; an extension rod 19 is fixedly connected to the end of the support spring 18; Rod 12 ends; The surface of the extension rod 19 is slidingly connected with the stirring rod 12; ...

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Abstract

The invention belongs to the technical field of L-amino acid synthesis, and particularly relates to a synthesis method of L-amino acid. The synthesis method comprises the following steps of S1, weighing a certain amount of soybean meal, adding distilled water in a solid-to-liquid ratio being 1 to 25, performing heat preservation in a water bath pot of 50 DEG C for 1h, adjusting pH to be 8.5, adding 10% of trypsin, performing hydrolysis for 4h, adding 8% of papain, performing hydrolysis for 4h, performing enzyme deactivation at 90 DEG C for 10min, performing cooling, performing standing, performing centrifugal separation to obtain supernatant, and preparing L-amino acid enzymolysis liquor; and S2, adding sodium selenite to the L-amino acid enzymolysis liquor in the mol mass proportion of L-amino acid to selenium salt being 2 to 1. According to the synthesis method disclosed by the invention, the L-amino acid and the selenium salt are synthesized and selenized to compound the L-amino acid, the products contain various amino acids needed by human bodies and contain biological active selenium, the selenium is easy to absorb under the action of amino acid carriers, double effects of complementing amino acids and complementing selenium for the human bodies can be achieved, and an opportunity of taking in various nutrient substances is provided for organisms.

Description

technical field [0001] The invention belongs to the technical field of L-amino acid synthesis, in particular to a synthesis method of L-amino acid. Background technique [0002] Amino acid is the basic unit of protein molecules in living organisms. It is closely related to life activities. It is an important material basis for the formation of organisms and life. It has special physiological functions and is one of the indispensable nutrients in living organisms. [0003] There are also some technical solutions for the synthesis of L-amino acids in the prior art. For example, a Chinese patent with application number 201810587713.0 discloses a production method of L-amino acids, which includes: using 1, ω-diamine or its The salt is an amino donor. In the presence of a substrate α-keto acid or its salt, transaminase is used to catalyze the transamination reaction between 1, ω-diamine and the substrate to obtain L-amino acid; the 1, ω-diamine The amine is 1,4-butanediamine, 1,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C12M1/00C12M1/02F26B5/06F26B11/12F26B25/06F26B25/04F26B25/08
CPCC12M23/02C12M27/06C12P13/04F26B5/06F26B11/12F26B25/04F26B25/06F26B25/08
Inventor 沈红超苏生鹏
Owner 沈红超
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