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Marker composition for premature ovarian failure gene detection and detection kit

A technology for premature ovarian failure and gene detection, which is applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc. Problems such as less research on correlation

Pending Publication Date: 2020-08-07
广州达康基因技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some genes and gene mutations related to premature ovarian failure have been found at home and abroad, so far, there are few studies on the correlation between different gene mutation sites of premature ovarian failure
The detection of single or individual genes cannot accurately and effectively reflect the situation of premature ovarian failure, and it is impossible to detect all the genes and loci that have been discovered. This not only has a huge workload, but also is inefficient, and it is difficult to meet the needs of clinical testing.
At present, there are no relatively complete studies and reports on genetic markers for premature ovarian failure.

Method used

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  • Marker composition for premature ovarian failure gene detection and detection kit
  • Marker composition for premature ovarian failure gene detection and detection kit
  • Marker composition for premature ovarian failure gene detection and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1 Materials and methods

[0025] 1.1 Materials

[0026] 1.1.1 Research object

[0027] (1) Premature ovarian failure case group: samples were collected from 192 premature ovarian failure outpatients of Xinhua Hospital from 2007 to 2008.

[0028] (2) Normal control group: 192 healthy volunteers from Shanghai Normal University and East China University of Science and Technology during the same period. Inclusion criteria: There is no history of premature ovarian failure, no ovarian cancer and other ovarian-related diseases among myself and my immediate family members within three generations.

[0029] These two groups are not related to each other.

[0030] 1.1.2 Main reagents

[0031] 1.1.2.1 DNA extraction reagents

[0032] Genomic DNA was extracted using a blood genomic DNA extraction kit purchased from QIAGEN.

[0033] 1.1.2.2 PCR reagents

[0034] (1) TaKaRa Ex Taq Hot Start Version: purchased from Treasure Bioengineering (Dalian) Co., Ltd., containing TaKaRa E...

Embodiment 2

[0127] This example illustrates the clinical application of the female premature ovarian failure gene detection marker combination model constructed in Example 1. Specifically, 192 Chinese female cases and 192 controls collected by Xinhua Hospital were used as research objects, and 47 mutation sites in 17 genes were genotyped, and the mainstream statistical method for studying the correlation of multiple mutation sites was used. , that is, multifactor dimensionality reduction (MDR) for statistical analysis, and initially established the BMP15, EIF2B2, ERCC6, ESR1, FIGLA, FOXL2, FSHR, HFM1, MCM8, MCM9, MSH5, NOBOX, NR5A1, POF1B, The female premature ovarian failure gene detection marker combination model composed of seventeen mutation sites of SGO2, STAG3 and SYCE1 has good detection efficiency of premature ovarian failure, OR=346.1179, P value<0.0001, accuracy 96.97%, sensitivity 98.92 %, specificity 100%. The establishment of a combination model of female premature ovarian f...

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Abstract

The invention discloses a marker composition for premature ovarian failure gene detection and a detection kit. The marker composition for premature ovarian failure gene detection is composed of 47 mutation sites of 17 genes including EIF2B2, ERCC6, ESR1, FIGLA, FOXL2, FSHR, HFM1, MCM8, MCM9, MSH5, NOBOX, NR5A1, POF1B, SGO2, STAG3 and SYCE1. According to the application, the marker composition forpremature ovarian failure gene detection is established for the first time, and a detection reagent and a kit are researched and developed according to the marker composition, so that the premature ovarian failure can be accurately, sensitively and specifically detected, therefore, the premature ovarian failure condition and risk can be known and judged more accurately and comprehensively, a reference basis is provided for early diagnosis, risk assessment and prognosis, and a new way and scheme are provided for clinical detection, pathogenesis and genetic research of premature ovarian failure.

Description

technical field [0001] The present application relates to the field of premature ovarian failure detection, in particular to a marker combination and a detection kit for premature ovarian failure gene detection. Background technique [0002] In recent years, premature ovarian failure (POF) has gradually become a common and frequently-occurring disease in gynecological clinics, and has received widespread attention and attention from all walks of life. The etiology of premature ovarian failure is complex, and its treatment is extremely difficult; its clinical manifestation is ovarian failure, which in turn leads to symptoms such as increased levels of follicle-stimulating hormone and decreased levels of estrogen, and finally leads to amenorrhea before the age of 40. [0003] Moreover, investigations and studies have shown that premature ovarian failure not only causes amenorrhea, infertility and other reproductive disorders, but also causes systemic vascular dysfunction, nigh...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118
Inventor 霍亮肖扬黎永祥
Owner 广州达康基因技术有限公司
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