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SNCG/SCC-Ag joint inspection colloidal gold test strip as well as preparation method and application thereof

A colloidal gold test paper and test strip technology, which is applied in the field of tumor marker detection, can solve the problems of increasing medical costs for patients, high maintenance costs, high costs of reagents and equipment, etc.

Pending Publication Date: 2020-08-04
BEIJING MODERN GAODA BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, chemiluminescent reagents and their supporting equipment are mainly used in large hospitals. There are also high cost of reagents and equipment, and high maintenance costs in the later stage, which will greatly increase the medical cost of patients.

Method used

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  • SNCG/SCC-Ag joint inspection colloidal gold test strip as well as preparation method and application thereof
  • SNCG/SCC-Ag joint inspection colloidal gold test strip as well as preparation method and application thereof
  • SNCG/SCC-Ag joint inspection colloidal gold test strip as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0109] The present invention also provides a kind of preparation method of SNCG / SCC-Ag joint detection colloidal gold test strip, described method comprises:

[0110] Preparation of colloidal gold particles;

[0111] The colloidal gold particles are combined with the first mouse anti-SNCG antibody and the first anti-SCC-Ag antibody to form a colloidal gold-labeled mouse anti-SNCG antibody / anti-SCC-Ag antibody colloidal gold conjugate;

[0112] The mouse anti-SNCG antibody / anti-SCC-Ag antibody colloidal gold conjugate is sprayed on the glass fiber membrane and dried to obtain a gold conjugate pad;

[0113] Prepare the detection line and the quality control line on the nitrocellulose membrane to obtain the reaction membrane, in which the solution containing the second mouse anti-SNCG antibody, the second anti-SCC-Ag antibody, and the solution containing the goat anti-mouse IgG polyclonal antibody were respectively Spray on nitrocellulose membrane to form SNCG detection line, SC...

Embodiment 1

[0136] In the following examples, unless otherwise specified, the substances used in the examples are commercially available products.

[0137] (1) Preparation of colloidal gold particles

[0138] Take 1.0g of chloroauric acid, equilibrate to room temperature, measure 2.0mL of purified water, put it into a glass bottle containing chloroauric acid, and blow repeatedly until the chloroauric acid is completely dissolved. Pour the dissolved auric acid into the container, rinse the glass bottle of auric acid with purified water for 5 times, and pour the washing solution back into the container. The solution was made up to 10.0 mL with purified water. Close the bottle cap tightly, shake fully for 15 minutes, mix well, and prepare 10% chloroauric acid solution.

[0139] Measure 0.3 mL of 10% chloroauric acid solution, add it into 100.0 mL of purified water, and stir until boiling while heating. Measure 0.6mL of 10% trisodium citrate solution, quickly add it to the boiling chloroau...

Embodiment 2

[0154] (1) Preparation of colloidal gold particles

[0155] The preparation steps are the same as in Example 1.

[0156] (2) Preparation of the first mouse anti-SNCG antibody and the second mouse anti-SNCG antibody

[0157] The preparation steps are the same as in Example 1.

[0158] (3) Preparation of colloidal gold-labeled mouse anti-SNCG antibody / anti-SCC-Ag antibody colloidal gold conjugate

[0159] The difference from Example 1 is that 1.8 mg of the first mouse anti-SNCG antibody prepared in step (2) and 2.0 mg of the first anti-SCC-Ag antibody were used. Other reaction conditions were the same as in Example 1.

[0160] (4) Preparation of gold conjugate pads

[0161] The difference from Example 1 is that after reconstitution, the content of the first mouse anti-SNCG antibody is 18 μg / mL, and the content of the first anti-SCC-Ag antibody is 20 μg / mL. 4.0 g Triton X-100 was used in the preparation of the colloidal gold conjugate resuspension. Other reaction conditions ...

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Abstract

The invention provides an SNCG / SCC-Ag joint inspection colloidal gold test strip. According to the test strip, a sample pad, a gold conjugate pad, a reaction film and an absorption pad are sequentially formed from a sample adding end, wherein the gold conjugate pad contains a first mouse anti-SNCG antibody and a first anti-SCC-Ag antibody, and the reaction film contains a second mouse anti-SNCG antibody and a second anti-SCC-Ag antibody. The SNCG / SCC-Ag joint inspection colloidal gold test strip prepared according to the invention not only can be used for completing conventional detection in hospitals, but also can be carried to home to complete detection, and compared with a colloidal gold test strip of a single antibody, the specificity and sensitivity of cancer detection can be improved, and the SNCG / SCC-Ag joint inspection colloidal gold test strip is more suitable for general screening of tumors.

Description

technical field [0001] The invention belongs to the field of tumor marker detection. Specifically, the invention relates to a colloidal gold test strip for detecting tumor gamma-synuclein (SNCG) / SCC-Ag, a preparation method and an application thereof. Background technique [0002] According to the data of "China Health and Family Planning Statistical Yearbook 2015", among the number of deaths caused by diseases in my country, cancer has become the first factor. Especially in the urban population, the number of deaths caused by cancer far exceeds that of heart disease at 27%. There are more than 10,000 new cases of cancer every day in the country. Therefore, cancer has become an important killer affecting the health of our country. However, when the cancer incidence rate in my country is comparable to the world level, the mortality rate is higher than the world level (the cancer mortality rate in China is 17% higher than the global average level). In addition to the fact th...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N33/558G01N33/532
CPCG01N33/6875G01N33/558G01N33/5748G01N33/57488G01N33/532
Inventor 唐小梅李晓楠荆志强韩晓亮王建铭
Owner BEIJING MODERN GAODA BIOTECHNOLOGY CO LTD
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