Method for quick identifying pear ring rot resistance

Abstract

The invention provides a method for quick identifying pear ring rot resistance, and belongs to the technical fields of plant disease resistance identification and evaluation and resource screening. The method comprises the steps of culturing pear ring rot pathogens, treating leaves, performing in vitro leaf inoculating and the like, in vitro leaves are inoculated with pear ring rot pathogens, andthe resistance of different species to the disease is identified through performing observing and comparing according to attack time and attack rate of the leaves of different species, inoculated withthe pathogens. The method has the advantage of early and repeated identification, the identification period of disease resistance,( identification for one batch only needs 6-8 days), is shortened, the identification result accuracy is improved, human resources and material resources for identification are saved, and high-flux identification is realized. In addition, the method is manually controllable in pathogenesis condition, the resistance identification results are accurate and consistent, and the method can scientifically, efficiently and accurately identify the pear ring rot resistance.

Description

technical field [0001] The invention belongs to the technical field of identification and evaluation of plant disease resistance and resource screening, and in particular relates to a method for rapidly identifying resistance to pear tree ring spot. Background technique [0002] Pear ring spot is one of the main diseases of pear trees in my country, which can cause exfoliation of branches, weakening of tree vigor, and fruit rot before and after harvesting. The occurrence and prevalence of this disease are closely related to meteorological conditions, the occurrence degree of branch ring disease, and the growth period of parasites. Current studies have shown that the disease is mainly transmitted through rainwater, causing large-scale disease in pear orchards and seriously endangering the yield and quality of pears. Since the main damage parts of pear ring spot are the branches and fruits of pear trees, it is not easy to observe the symptoms of the pathogen during the incuba...

AI Technical Summary

Problems solved by technology

The results of resistance identification in disease nurseries are easily affected by the density distribution of regional bacterial colonies and site environmental factors. There will be great differences in the identification results at different identification times, and it is also difficult to conduct high-throughput resistance identification

[0004] Field inoculation identification is also a field identification method, which solves the limitations of disease nursery identification to a certain extent, but is still affected by the age of the inoculated tree, planting environment, and climatic conditions, and the error is large, and it is not easy to observe the occurrence time of pear branch diseases, The process and the degree of disease, the operation is complicated, the cost is high, and the accuracy is poor

The field identification test generally covers a large area, the identification cycle is long, requires a lot of manpower to handle and manage, and the test cost is high

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