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Intestinal-tract-derived flora for diagnosing sarcopenia and use of intestinal-tract-derived flora

The technology of a use and a diagnostic kit, which is applied in the field of flora in the diagnosis of sarcopenia, can solve the problems of inability to achieve early warning and non-invasive diagnosis, and achieve high accuracy, high sensitivity, and reduced prevalence Effect

Active Publication Date: 2020-07-24
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the deficiencies of the prior art, the purpose of the present invention is to provide intestinal flora related to the occurrence and development of sarcopenia and its application in the diagnosis and treatment of sarcopenia, so as to overcome the inability to achieve early diagnosis of sarcopenia Early warning, failure to achieve non-invasive diagnosis, etc., can help disease diagnosis and guide drug research, precision medicine, etc.

Method used

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  • Intestinal-tract-derived flora for diagnosing sarcopenia and use of intestinal-tract-derived flora
  • Intestinal-tract-derived flora for diagnosing sarcopenia and use of intestinal-tract-derived flora
  • Intestinal-tract-derived flora for diagnosing sarcopenia and use of intestinal-tract-derived flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Screening of intestinal flora associated with sarcopenia

[0053] 1. Research objects and sample collection

[0054] Stool samples were collected from 11 sarcopenic and 60 healthy individuals. The patient statistics are shown in Table 1.

[0055] Table 1 Patient Information

[0056]

[0057] 2. 16S rRNA sequencing

[0058] 2.1 Extraction of DNA

[0059] Genomic DNA was extracted from the samples using the Genomic DNA Extraction Kit, and the operation steps were carried out according to the instructions.

[0060] 2.2 DNA sample purity and concentration determination

[0061] Genomic DNA was detected by 1% agarose gel electrophoresis.

[0062] 2.3PCR amplification and product purification

[0063] According to the designated sequencing region, synthesize specific primers with barcode, or synthesize fusion primers with misplaced bases.

[0064] PCR used TransGen AP221-02: TransStart Fastpfu DNAPolymerase; all samples were carried out according to the f...

Embodiment 2

[0083] Example 2 Verifies the correlation between microbial markers and sarcopenia

[0084] 1. Research object and sample collection

[0085] According to the method of Example 1, 11 samples of sarcopenia and 72 samples of healthy people were collected. The patient statistics are shown in Table 3.

[0086] Table 3 Patient Information

[0087]

[0088] 2. 16S rRNA sequencing and data analysis are the same as in Example 1.

[0089] 3. Results

[0090] The test results showed that the abundance levels of Oscillibacter and Eubacterium_brachy_group were significantly increased and the difference was statistically significant (Oscillibacter: P=0.01458; Eubacterium_brachy_group: P=0.014354). The results of the ROC curve showed that the AUC value of Oscillibacter as a detection variable was 0.821, the specificity of the optimal critical value was 0.861, and the sensitivity was 0.727 ( image 3 A); The AUC value of the Eubacterium_brachy_group detection variable is 0.732, the spe...

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Abstract

The invention discloses an intestinal-tract-derived flora for diagnosing sarcopenia and use of the intestinal-tract-derived flora, and belongs to the biomedicine field. The flora contains Oscillibacter and Eubacterium_brachy_group. By detecting intestinal flora markers, the risk that a subject suffers from the sarcopenia can be evaluated, and auxiliary references are provided for diagnosis and treatment of diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the intestinal flora for diagnosing sarcopenia and its application. Background technique [0002] Sarcopenia, referred to as sarcopenia, was first proposed by Evans WJ and Rosenberg IR in 1991. It is a condition characterized by skeletal muscle loss, and also involves mitochondrial dysfunction, skeletal muscle inflammation, hormone A series of symptoms caused by disorder and other reasons (Lang T, Streeper, Cawthon P, Baldwin K, Taaffe DR, Harris TB. Sarcopenia: etiology, clinical consequences, intervention, and assessment [J]. Osteoporos Int, 2010, 21 (4 ):543-559.). Due to the increasingly serious problem of population aging, sarcopenia, as a common disease of the elderly, is a major threat to public health. From the age of 25, the number of skeletal muscle fibers in the human body decreases every year. By the age of 70, the body's muscle volume decreases by 40%, muscle strength de...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6869C12Q1/06C12R1/01
CPCC12Q1/6869C12Q1/689C12Q2600/158C12Q2600/178C12Q2531/113C12Q2525/191C12Q2563/107
Inventor 康琳
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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