Method for identifying cold resistance of grapes based on frost climate chamber-greenhouse sand storage culture

Abstract

The present invention relates to a method for identifying the cold resistance of grapes based on frost climate chamber-greenhouse sand storage culture. The method comprises the steps of S1, collectingnormally-growing and disease-free annual mature branches as test branches on January 5 to 10; S2, putting all the test branches into a frost climate chamber, and performing dark environment treatmentfor 10-12 hours under the conditions that the temperature is -24 DEG C to -26 DEG C and the relative humidity is 60-65%; S3, transferring the test branches treated with the frost climate chamber intoa sunlight greenhouse for sand storage culture, horizontally burying the test branches into the position of 10-12 cm below the surface of a sand pit, performing complete watering once, covering the surface of the sand pit by a light-pervious plastic mulching film, controlling the relative humidity and the temperature of the position of 10-12 cm below the surface of the sand pit to be not lower than 60% and 15-20 DEG C, and enabling the sand pit to be exposed to light from 7:30-8:30 a.m. to 14:30-15:30 p.m. each day; and S4, identifying the cold resistance, wherein the test branches are takenout after sand storage culture of 35-40 days, and the germination rate is subjected to statistics and serves as a judgment index of the cold resistance. The frost climate chamber and the greenhouse are combined for sand storage culture, and by investigating the germination rate of the branches, the cold resistance of the ultra-large-population (ten thousand or above) grapes is quickly and accurately identified.

Description

technical field [0001] The invention relates to the field of identification of cold resistance of tree species, in particular to a method for identifying cold resistance of grapes based on sand storage culture in a frost climate chamber-greenhouse. Background technique [0002] In order to meet the market demand, Zao grapes will be launched in May. Most areas in my country belong to the temperate climate, and there may be cold weather such as snow and frost during February to April, and grape varieties with excellent cold resistance can successfully survive the cold weather in late spring. In addition, the old roots of grapes also need to survive the winter. In northern my country, the climate is very cold in winter, and the cold resistance determines the germination rate of old roots of grapes and the harvest of grapes in the coming year. The cold resistance of grapes has high heritability, and the cold resistance ability of different grape resources is significantly diffe...

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Problems solved by technology

[0003] However, in the actual measurement, we found that due to factors such as ambient temperature, sample soaking time, heating time, and the concentration of CO2 in the air and its solubility in water, it will affect the actual measured conductivity. Poor repeatability of results and inaccurate identification results

The main limitation is that the identification method of electrical conductivity in the laboratory is very complicated to operate, and it will be a huge project for identifying the cold resistance of thousands of different grape varieties. A lot of labor, and not very intuitive

Therefore, the existing identification methods cannot quickly and accurately identify the cold resistance of grapes in large groups.

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