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Induced tetraploid paulownia subculture propagation-expanding efficient culture medium formula and application thereof

A high-efficiency multiplication and culture medium technology, applied in the biological field, can solve the problems of low propagation coefficient and cannot meet the needs of large-scale seedling raising, and achieve the effects of improving seedling raising efficiency, saving manpower and shortening the cultivation period.

Active Publication Date: 2020-07-14
SHANDONG FOREST SCI RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Our laboratory has used the formula reported in the literature to conduct tissue culture experiments, and the multiplication coefficient is very low, which is far from meeting the needs of large-scale seedling cultivation. This may be due to the fact that the tetraploid paulownia has not been industrialized using tissue culture technology for more than 10 years. Key Reasons for Nurturing Seedlings

Method used

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  • Induced tetraploid paulownia subculture propagation-expanding efficient culture medium formula and application thereof
  • Induced tetraploid paulownia subculture propagation-expanding efficient culture medium formula and application thereof
  • Induced tetraploid paulownia subculture propagation-expanding efficient culture medium formula and application thereof

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Experimental program
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Effect test

Embodiment 1

[0023] Cut the sterile seedlings into about 2cm long bud stem sections on the ultra-clean workbench, and transfer them to the high-efficiency proliferation medium MS+IAA 0.05mg / L+6-BA 0.5mg / L+KT 0.05mg / L+ NAA 0.05mg / L+Spd 0.05mg / L+agar 4.5g / L+sucrose 30g / L (pH 5.8), the culture conditions were daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After 25 days, clustered buds were induced to form, and the multiplication factor was 15-20 times, see image 3 .

Embodiment 2

[0025]Cut the sterile seedlings into about 2cm long bud stem sections on the ultra-clean workbench, and transfer them to the high-efficiency proliferation medium MS+IAA 0.5mg / L+6-BA 4.0mg / L+KT 0.5mg / L+ NAA 0.5mg / L+Spd 0.5mg / L+agar 4.5g / L+sucrose 40g / L (pH 5.8), culture conditions were daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After 25 days, clustered shoots were induced to form, and the multiplication factor was 30-40 times, see Figure 4 .

Embodiment 3

[0027] Cut the sterile seedlings into about 2cm long bud stem sections on the ultra-clean workbench, and transfer them to the high-efficiency proliferation medium MS+IAA 5.0mg / L+6-BA 14.0mg / L+KT 5.0mg / L+ NAA 5.0mg / L+Spd 5.0mg / L+agar 4.5g / L+sucrose 50g / L (pH 5.8), culture conditions were daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After 25 days, clustered buds were induced to form, and the multiplication factor was 16-23 times, see Figure 5 .

[0028] image 3 , Figure 4 with Figure 5 For the clustered buds regenerated after 25 days of culture using the medium formula of the present invention, the multiplication factor reaches 15-40 times.

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Abstract

The invention discloses an induced tetraploid paulownia subculture propagation-expanding efficient propagation culture medium formula and an application thereof. An aseptic seedling is cut into stem segments with buds to be transferred to a high-efficient proliferation culture medium; the high-efficiency proliferation culture medium is composed of an MS culture medium added with 0.05-5.0 mg / L of indoleacetic acid, 0.5-14.0 mg / L of 6-benzylamino adenine, 0.05-5.0 mg / L of kinetin, 0.05-5.0 mg / L of naphthylacetic acid, 0.05-5.0 mg / L of spermidine, 4.0-6.0 g / L of agar and 30-50g / L of saccharose,wherein the culture condition is that the temperature in the day time is 25 DEG C, the temperature at night is 18 DEG C, the illumination intensity is 2500 Lx, and the illumination time is 13 h / d. After culture of 20 days, the proliferation times can reach as high as 30-40 times. The tetraploid paulownia efficient proliferation is dramatically promoted, the breeding period is effectively shortenedand the breeding efficiency is improved. Technical support is provided for industrialized production of tetraploid paulownia excellent lines, and the problem of tetraploid paulownia excellent varietyseedlings are in short supply is solved.

Description

technical field [0001] The invention relates to a formulation and application of a high-efficiency propagation medium for inducing tetraploid paulownia subculture and multiplication, which is especially suitable for subgeneration and multiplication in the early stage of industrial seedling cultivation, and belongs to the field of biotechnology. Background technique [0002] Paulownia is a plant of the genus Paulownia in the family Scrophulariaceae. It is native to my country. It has no flying catkins, beautiful trees, and a long history of cultivation. It is naturally distributed in 25 provinces, municipalities, and autonomous regions in my country. It is an important fast-growing timber and native tree species for landscaping in my country. The leaves, flowers, fruits and bark of Paulownia can be used as medicine, and the leaves and flowers of Paulownia can be used as feed. Paulownia wood is high-quality and high-grade, corrosion-resistant, acid and alkali resistant, wear-r...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 毛秀红王迎毛欣李猛闫少波亓玉昆华辉刘泉仲伟国梁燕王晓英杜辉
Owner SHANDONG FOREST SCI RES INST
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