Water quality microorganism indication method based on big data and molecular biotechnology

Abstract

The invention provides a water quality microorganism indication method based on big data and a molecular biotechnology. The method comprises the following steps: (1) collecting samples in a research area, detecting water quality, and analyzing main pollution factors and water quality characteristics of the research area; (2) constructing a water quality comprehensive score capable of reflecting the water quality condition to obtain a water quality comprehensive score judgment standard, and determining the water quality grades of all the samples; (3) obtaining a microbial flora structure of theresearch area through high-throughput sequencing, and screening out indicative microorganisms capable of indicating water quality change based on bioinformatics analysis; and (4) designing a specificprimer, constructing a fitting equation of the relative abundance of the indicative microorganisms and the comprehensive score of the water quality, and predicting the water quality grade of a sampleaccording to the relative abundance of the indicative microorganisms. The invention provides a technology for indicating the water quality of a water environment by using planktonic bacteria in a water body. The screened indicative microorganisms can be widely applied to water quality indication and evaluation of various water environments, and powerful support is provided for pollution prevention and control.

Description

technical field [0001] The invention relates to the technical field of water environment water quality detection and evaluation, in particular to a water quality microbial indication method based on big data and molecular biotechnology. Background technique [0002] The drinking water sources of many cities come from surface water bodies, including various rivers, lakes, reservoirs, etc. In recent years, with the continuous development of industrial and agricultural production, various exogenous pollutants have entered the water environment through point source discharge, surface source inflow or ship movement risk source discharge. While these pollutants cause water environment pollution, they also seriously threaten the safety of drinking water and urban centralized water supply. Therefore, it is of great significance to strengthen the rapid and efficient detection and early warning technology research and development of water environment and water quality to ensure the n...

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Problems solved by technology

On the one hand, it increases the operation time and cost, on the other hand, it requires higher professional quality of the staff

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