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Method for extracting monoterpene glycoside type effective components from nonmedicinal parts of paeonia lactiflora

A technology of medicinal parts and monoterpene glycosides is applied in the field of extraction of effective components of traditional Chinese medicine, which can solve the problems of extraction and have not yet seen effective components, and achieve the effect of reducing potential toxic components and enriching and effectively comprehensive utilization.

Inactive Publication Date: 2020-07-10
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it still contains active ingredients such as oxidized paeoniflorin, paeoniflorin, paeoniflorin, galloyl paeoniflorin, benzoyl paeoniflorin, etc., but there are also some potentially toxic ingredients such as benzoic acid. Research reports on the extraction of active ingredients from these non-medicinal parts

Method used

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  • Method for extracting monoterpene glycoside type effective components from nonmedicinal parts of paeonia lactiflora
  • Method for extracting monoterpene glycoside type effective components from nonmedicinal parts of paeonia lactiflora
  • Method for extracting monoterpene glycoside type effective components from nonmedicinal parts of paeonia lactiflora

Examples

Experimental program
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Effect test

Embodiment 1

[0185] This example aims to illustrate a method for extracting active ingredients from the non-medicinal parts of Radix Paeoniae Alba, the specific steps are as follows:

[0186] (1) Dry and pulverize the non-medicinal parts of Radix Paeoniae Alba, pass through a 10-mesh sieve to obtain a granular sample;

[0187] (2) Using 12 times the volume of 50% ethanol, heat and reflux extraction twice, each extraction time is 80min, combine the extracts, filter and concentrate to obtain a crude extract with a concentration of 0.25-0.5g dry raw materials / mL;

[0188](3) Adopt a chromatographic column with a column diameter-to-height ratio of 1:4, use D-101 macroporous resin as a filler, load 3BV of the crude extract at a loading speed of 0.5mL / min, and then use 2 times volume of water and 4 times the volume of 50% ethanol to elute the macroporous resin chromatography column at an elution rate of 0.75mL / min, collect the eluate from the elution site of 50% ethanol, concentrate, and dry to ...

Embodiment 2

[0190] The difference between this example and Example 1 is that 12 times the volume of 50% ethanol in step (2) is replaced by 10 times the volume of 70% ethanol. Precisely weigh the refined extract dried to constant weight, and carry out content determination according to the above method, then the content of each component is as follows: paeoniflorin 38.9%, paeoniflorin 17.6%, benzoyl paeoniflorin 1.85%, galloyl paeoniflorin Glycoside 1.45%, oxidized paeoniflorin 0.34%, gallic acid 0.045%, benzoic acid 1.5%, and the total content of monoterpene glycosides in the effective part is 60.14%. The yield of purified products in this process is 7.2%, so the transfer rate of monoterpene glycosides is 75.5%, the transfer rate of gallic acid is 0.40%, and the transfer rate of benzoic acid is 46.2%.

Embodiment 3

[0192] The difference between this example and Example 1 is that in step (3), the macroporous resin chromatography column is eluted with 2 times the volume of water and 4 times the volume of 50% ethanol at an elution rate of 1 mL / min. Precisely weigh the refined extract dried to constant weight, and carry out content determination according to the above method, then the content of each component is as follows: paeoniflorin 38.64%, paeoniflorin 17.5%, benzoyl paeoniflorin 1.85%, galloyl paeoniflorin Glycoside 1.93%, oxidized paeoniflorin 0.33%, gallic acid 0.033%, benzoic acid 1.1%, and the total content of monoterpene glycosides in the effective part is 60.25%. The yield of purified products in this process is 7.2%, so the transfer rate of monoterpene glycosides is 74.7%, the transfer rate of gallic acid is 0.29%, and the transfer rate of benzoic acid is 33.8%.

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Abstract

The invention relates to extraction of effective components of traditional Chinese medicines and in particular discloses a method for extracting monoterpene glycoside type effective components from nonmedicinal parts of paeonia lactiflora. The method comprises the following steps: drying and crushing nonmedicinal parts of paeonia lactiflora so as to obtain a granule sample; performing extraction on the granule sample by using water or an alcohol-water mixed solvent so as to obtain a crude extract; and adsorbing a loading buffer, which is prepared from the crude extract, by using a macroporousresin, further performing gradient elution by using ethanol-water mixed solvents as eluants with 0% and 50% ethanol, collecting an elution liquid of an elution part with 50% ethanol, and performing concentration and drying, so as to obtain a refined extract. By adopting the method provided by the invention, not only multiple monoterpene glycoside components effectively enriched, but also potentialtoxic components are reduced, and effective comprehensive utilization of traditional Chinese medicine resources are expanded and enriched.

Description

technical field [0001] The invention relates to the extraction of effective components of traditional Chinese medicines, in particular to a method for extracting active components of monoterpene glycosides from non-medicinal parts of Radix Paeoniae Alba. Background technique [0002] The active ingredients of Radix Paeoniae Alba are monoterpene glycosides of peony, mainly including oxidized paeoniflorin, paeoniflorin, paeoniflorin, galloyl paeoniflorin and benzoyl paeoniflorin, each of which has a variety of pharmacological effects and uses. At present, it is mainly extracted from the taproot of Paeoniae Alba. [0003] Most of the technologies related to the extraction or preparation of monoterpene glycosides from Paeoniae alba and other Paeoniaceae plants are mainly concentrated on the two main components of paeoniflorin and paeonifloride, and the focus is on obtaining some monomer components. The process is relatively complicated. Whether the obtained monomer components a...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K36/65A61K125/00
CPCA61K36/65A61K36/71A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 龚慕辛马喆谢凯莉
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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