Method for detecting ciprofloxacin in milk

Abstract

The invention discloses a method for detecting ciprofloxacin in milk, and the method comprises the following steps: carrying out deproteinization operation on a milk sample to obtain a solution to bedetected; placing the surface enhancing reagent in a container; putting the to-be-detected liquid into a container; putting the flocculating agent into a container; uniformly mixing the solution in the container to obtain a mixed solution; performing Raman spectrum scanning on the mixed solution to obtain a Raman spectrum of the mixed solution, and determining whether ciprofloxacin exists in the milk or not based on the characteristic peak of the obtained Raman spectrum. According to the method, a surface enhancing reagent and a flocculating agent are adopted as SERS enhancing reagents, the SERS effect of ciprofloxacin in milk can be enhanced, stronger ciprofloxacin Raman signals are obtained, the detection sensitivity of ciprofloxacin in milk is greatly improved, and the lower detection limit of ciprofloxacin is low.

Description

technical field [0001] At least one embodiment of the present disclosure relates to a method for detecting ciprofloxacin in milk, in particular, to a method for detecting ciprofloxacin in milk. Background technique [0002] Ciprofloxacin is a quinolone veterinary drug, which is often added to the feed of food, livestock and poultry animals to reduce the incidence of animal diseases. Excessive ciprofloxacin in feed may remain in downstream food, and eating a large amount of food with high ciprofloxacin residue will pose a threat to human health. Therefore, it is very important to establish a detection method for ciprofloxacin in food to control its residue and protect human health. [0003] Milk is a food that may contain ciprofloxacin residues. The detection methods for ciprofloxacin in milk matrix are divided into laboratory methods and rapid detection methods. The laboratory method is mainly liquid chromatography-tandem mass spectrometry, see GB / T22985-2008 Enrofloxacin,...

AI Technical Summary

Problems solved by technology

The laboratory method is mainly liquid chromatography-tandem mass spectrometry, see GB / T22985-2008 Enrofloxacin, danofloxacin, ciprofloxacin, sarafloxacin, orbifloxacin, difluoro The liquid chromatography-tandem mass spectrometry method for the determination of the residues of Floxacin and Mabofloxacin is characterized by high sensitivity and accurate quantification, but it has the limitations of tedious and time-consuming experimental process, large and expensive equipment, and it is difficult to quickly screen on the spot Promote the application in the scene

[0004] The rapid detection methods mainly include the method based on colloidal gold and the method based on surface-enhanced Raman spectroscopy (SERS). Among them, the colloidal gold method has the characteristics of high sensitivity, but the cost is relatively high and cannot be quantified (Wan Yuping, Zhao Zhengmiao , Tian Tian, ​​Wang Shanliang, Han Jingpeng. Colloidal gold immunochromatography for rapid detection of fluoroquinolone residues in milk [J]. China Dairy Industry, 2012(12): 50-54.)

However, the commonly used method based on surface-enhanced Raman spectroscopy (SERS) has the disadvantages of low detection sensitivity and the inability to detect the low content of ciprofloxacin in milk.

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