Micro-fluidic chip and preparation method thereof and preparation method of unicellular micro-droplet

Abstract

The invention provides a micro-fluidic chip. The micro-fluidic chip comprises a chip body and a confluence channel, wherein an oil phase inlet, a micro-bead solution inlet, a cell suspension inlet anda micro-droplet outlet are formed in the chip body; the oil phase inlet, the micro-bead solution inlet and the cell suspension inlet are inlets for enabling droplet generation oil, a micro-bead solution and cell suspension to enter the chip body in sequence; the cell suspension contains a plurality of cells; the micro-bead solution contains a plurality of micro-beads; the confluence channel is communicated with the micro-droplet outlet and the oil phase inlet; the micro-fluidic chip further comprises a first fluid control unit for communicating the micro-bead solution inlet with the confluence channel and a second fluid control unit for communicating the cell suspension inlet with the confluence channel; the first fluid control unit and the second fluid control unit are used for arrayingthe micro-beads or the cells according to a certain sequence; the micro-bead solution is firstly converged with the cell suspension to form laminar flow which is not mutually mixed; and the convergedlaminar flow is converged with the droplet generation oil to form micro-droplets and the micro-droplets are output from the micro-droplet outlet. The micro-fluidic chip provided by the invention has ahigh single-package rate.

Description

technical field [0001] The invention relates to the field of microfluidic technology and related devices, in particular to a microfluidic chip, a method for preparing the microfluidic chip, and a method for preparing single-cell microdroplets. Background technique [0002] Microfluidic chip, also known as lab-on-a-chip, can integrate conventional biochemical reactions into a chip of a few square centimeters. Because microfluidic chips have micron-scale channels, which are comparable to the size of cells, they have attracted more and more attention in cell research. [0003] However, the microfluidic chips currently on the market have complex structures, complex manufacturing processes, and high manufacturing costs. The droplets generated by some microfluidic chips are easy to wrap multiple particles (such as microbeads or cells) or contain no particles. These phenomena will increase the cost of the experiment, reduce the efficiency of the experiment and lead to the problem ...

AI Technical Summary

Problems solved by technology

[0003] However, the microfluidic chips currently on the market have complex structures, complex manufacturing processes, and high manufacturing costs.

The droplets generated by some microfluidic chips are easy to wrap multiple particles (such as microbeads or cells) or contain no particles. These phenomena will increase the cost of the experiment, reduce the efficiency of the experiment and lead to the problem of low encapsulation rate.

In addition, the throughput of microfluidic chips on the market is low

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