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Method for detecting copy number variation of sheep ZNF280BY gene, and application of method

A gene copy number and copy number technology, which is applied in the field of molecular genetics detection, can solve the undiscovered problems such as the correlation of ZNF280BY gene copy number variation and reproductive traits, and achieve the effect of low cost, easy operation, simple and fast operation

Active Publication Date: 2020-06-30
甘肃润牧生物工程有限责任公司
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Problems solved by technology

However, so far, no reports have been found on the detection of sheep ZNF280BY gene copy number variation and its correlation with gene expression and reproductive traits

Method used

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  • Method for detecting copy number variation of sheep ZNF280BY gene, and application of method
  • Method for detecting copy number variation of sheep ZNF280BY gene, and application of method
  • Method for detecting copy number variation of sheep ZNF280BY gene, and application of method

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Embodiment Construction

[0032] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments. The examples are only used to explain the present invention, not to limit the protection scope of the present invention.

[0033] The present invention uses real-time quantitative PCR technology to detect and analyze the ZNF280BY gene copy number variation and gene expression of the sheep Y chromosome, mainly including the following steps: using the DDX3Y gene and β-actin gene that have been verified as single copies by sequencing as reference, and using real-time Quantitative PCR was used to identify the copy number and expression level of ZNF280BY gene.

[0034]In order to improve the accuracy of the results, strict experimental operations are carried out according to the requirements of standardized real-time quantitative PCR operations, including: ensuring the extraction of high-quality genomic DNA and RNA, conducting strict target gene primer desig...

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Abstract

The invention discloses a method for detecting copy number variation of a sheep ZNF280BY gene, and an application of the method. According to the method, full genome DNA of a ram to be detected is taken as a template, and partial fragments of a ZNF280BY gene and a DDX3Y gene of the ram are respectively amplified by using a male specific qPCR primer pair P1 and P2, wherein the multi-copy ZNF280BY gene is used as a detection gene, the single-copy DDX3Y gene is used as a reference gene, and the copy number of the ram ZNF280BY gene is calculated according to a quantitative result. The method can be used to detect a copy number variation molecular marker used for early identification of breeding capacity of rams, so that the breeding process of the rams is accelerated through molecular marker-assisted selection. The method is simple and rapid, and is convenient for popularization and application.

Description

technical field [0001] The invention belongs to the field of molecular genetics detection, and relates to a method for detecting the copy number variation of sheep Y chromosome ZNF280BY gene (Zincfinger protein 280B, Y-linked). ) as a reference, the absolute quantification method of the calibration sample was used to calculate the copy number of the ZNF280BY gene in the ram sample, so as to speed up the ram breeding process according to the molecular markers obtained from the detection that can early identify the ram fecundity. Background technique [0002] With the continuous in-depth study of genome, genome structure variation has been paid more and more attention by scholars. The Y chromosome is a male-specific chromosome and plays an important role in genetic breeding. Many types of polymorphisms have been found on the Y chromosome: single nucleotide polymorphism, insertion / deletion, short tandem repeat on the Y chromosome (STR) and gene copy number variation (Copy numb...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851
CPCC12Q1/6888C12Q1/6851C12Q2600/156C12Q2600/124C12Q2600/166C12Q2531/113C12Q2537/16C12Q2545/101
Inventor 乐祥鹏徐海月李发弟王丽
Owner 甘肃润牧生物工程有限责任公司
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