Application of Trichoderma in Resisting Soybean Quarantine Diseases

A technology of Trichoderma and soybean, applied in application, chemicals for biological control, fungi, etc., can solve problems such as cumulative poisoning, environmental pollution, pesticide residues, etc.

Active Publication Date: 2020-12-11
中华人民共和国京唐港海关
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical reagents, on the one hand, causes pesticide residues and pollutes the environment. According to literature reports, the utilization rate of pesticides is generally 10%, and about 90% of them remain in the environment, causing pollution to the environment; on the other hand, pesticides can harm human health. After entering the human body through oral or contact, it can cause acute poisoning in a short period of time, or gradually accumulate in the food chain, causing cumulative poisoning after human consumption

Method used

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  • Application of Trichoderma in Resisting Soybean Quarantine Diseases
  • Application of Trichoderma in Resisting Soybean Quarantine Diseases
  • Application of Trichoderma in Resisting Soybean Quarantine Diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Put Trichoderma harzianum and southern stem canker of soybean on the PDA medium, culture in the incubator at 25°C for 5 days, punch holes with a puncher, punch the bacteria into 0.5cm in diameter, and pick one piece respectively Bacterial blocks were placed in the same PDA petri dish, placed in a straight line opposite to each other, with an interval of about 6 cm. Finally, the petri dish was placed in a 25°C incubator for cultivation, and the growth status of the colony was observed every 24 hours.

[0035] The result is as figure 1 As shown, after 3 days, the contact between Trichoderma harzianum and southern stem canker soya bean can be seen. In the following observations, it was found that the two strains were inhibited after meeting, and the growth rate slowed down. After 5 days, the contact part had obvious The atrophy traces, the contact edge has a clear isolation zone, and Trichoderma harzianum forms an encirclement around the southern stem canker of soybean. T...

Embodiment 2

[0037] Put Trichoderma harzianum and soybean seed rot fungus on PDA medium, culture them in an incubator at 25°C for 5 days, use a puncher to make holes, punch the fungi into 0.5cm diameter bacteria blocks, and then pick a piece of bacteria respectively The blocks were placed in the same PDA petri dish in a straight line with an interval of about 6 cm. Finally, the petri dish was placed in a 25°C incubator for cultivation, and the growth status of the colony was observed every 24 hours.

[0038] The result is as figure 2 As shown, two strains of Trichoderma harzianum and soybean seed rot can be seen to be in contact after 3 days. In subsequent observations, it was found that the two strains were inhibited after meeting, and the growth rate slowed down. After 5 days, the contact part had obvious There are signs of shrinkage, and there is an obvious isolation zone at the contact edge, and Trichoderma harzianum forms an encirclement circle around soybean seed rot fungus. Theref...

Embodiment 3

[0040] Put Trichoderma viride and southern stem canker of soybean on the PDA medium, culture in the incubator at 25°C for 5 days, punch holes with a puncher, punch the bacteria into 0.5cm in diameter, and then pick one piece respectively Bacterial blocks were placed in the same PDA petri dish, placed in a straight line opposite to each other, with an interval of about 6 cm. Finally, the petri dish was placed in a 25°C incubator for cultivation, and the growth status of the colony was observed every 24 hours.

[0041] The result is as image 3 As shown, after 3 days, it can be seen that the two strains of Trichoderma viride and southern stem canker soya bean can be in contact with each other. In subsequent observations, it is found that the two strains are inhibited after they meet, and the growth rate slows down. After 5 days, the contact part has obvious There are obvious atrophy marks on the contact edge, and Trichoderma viride forms an encirclement around southern stem cank...

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Abstract

The invention provides an application of trichoderma in antagonizing soybean quarantine diseases. The invention relates to the technical field of biocontrol agents. In the embodiment, trichoderma harzianum, trichoderma viride and trichoderma spiroides are separated and utilized; bactericidal tests on Diaporthe phaseolorum caulivora and Phomopsis longicolla are carried out; it is shown by test results that trichoderma harzianum, trichoderma viride and trichoderma spirale have good antibacterial effects and antagonistic effects on Diaporthe phaseolorum caulivora and Phomopsis longicolla, and canbe used as standard strains for biological prevention and treatment.

Description

technical field [0001] The invention belongs to the technical field of biocontrol fungi, and in particular relates to the application of Trichoderma in antagonizing soybean quarantine diseases. Background technique [0002] In the List of Imported Plant Quarantine Pests of the People's Republic of China, Diaporthe phaseolorum var. meridionalis and Phomopsis longicolla are the quarantine pests most frequently intercepted at ports of imported soybeans in my country. pests. According to customs statistics, 230 batches of Soybean southern stem canker and 7,036 batches of Soybean stem canker have been intercepted from imported soybeans since 2006. [0003] Soybean southern stem canker is one of the 13 quarantine pathogens on soybeans that my country has focused on. It was first reported in the southern United States in 1973, and then it commonly occurred in the southern states of the United States, and the loss rate was as high as 100% in the severely affected areas. The pathoge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00
Inventor 李海云陈寅宋宁宁谢海翠柳吉芹田虎范光辉张建新
Owner 中华人民共和国京唐港海关
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