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Ammonia water combined with ultrasonic extraction method and system for polyhydroxyalkanoate

A polyhydroxyalkanoate and ultrasonic technology, which is applied in the field of ammonia water combined with ultrasonic extraction of polyhydroxyalkanoates, can solve the problems of high production cost, low PHA polymerization degree, low PHA purity and yield, etc. The effect of mitigating degradation

Active Publication Date: 2022-04-08
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the high production cost of polyhydroxyalkanoate (PHA) existing in the prior art, the polymerization degree of the extracted PHA is low, and the problem that the purity and yield of PHA are low, provide a kind of ammonia water combination A method and system for ultrasonically extracting polyhydroxyalkanoate, in order to achieve the purpose of extracting and obtaining PHA with a higher degree of polymerization, and the purity and yield of the obtained PHA are higher, and the purpose of reducing the production cost of PHA

Method used

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  • Ammonia water combined with ultrasonic extraction method and system for polyhydroxyalkanoate

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Embodiment approach

[0067] In the present invention, the fermentation broth of the polyhydroxyalkanoate can be the fermentation broth of microorganisms that can be used to prepare polyhydroxyalkanoate conventionally in the art. Preferably, the microorganism is a halophilic bacterium, for example, it can be a salt One of the genus Monas, according to a preferred embodiment of the present invention, the PHA fermentation strain is Halomonas sp.; more preferably, the PHA fermentation strain is Halomonas sp. Bacteria (Halomonas sp.) TD01, its preservation number is CGMCC NO.4353 (CN201010578858.8).

[0068] In the present invention, the fermentation medium used for the fermentation of the polyhydroxyalkanoate is not particularly limited, and may be a conventionally used fermentation medium in the art, and will not be repeated here.

[0069] In the present invention, the conditions for the fermentation of the polyhydroxyalkanoate are not particularly limited, and may be conventionally used fermentation...

preparation example 1

[0104] Halomonas (TD01, whose preservation number is CGMCC NO.4353 (CN201010578858.8)) was inoculated in the seed medium (yeast powder of 5g / L, peptone of 10g / L and sodium chloride of 60g / L) Under the conditions of 37°C and 200rpm, carry out primary activation culture, culture to OD 600 Reach about 4, obtain first-grade seed liquid;

[0105] Inoculate the primary seed liquid into the seed medium with an inoculation amount of 10% by volume, carry out secondary activation culture at 37°C and 200rpm, and cultivate to OD 600 Reach about 4, obtain secondary seed liquid, obtain fermented seed liquid.

[0106] Then the inoculum of 10 volume % is inserted into the initial fermentation medium (sodium chloride 50g / L, glucose 50g / L, corn steep liquor powder 15g / L, urea 2g / L, magnesium sulfate 0.2g / L) L, potassium dihydrogen phosphate 5g / L, trace element mother liquid I 10mL / L, trace element mother liquid II 3mL / L. The trace element mother liquid I and II refer to the cited patent CN201...

Embodiment 1

[0108] (1) The polyhydroxyalkanoate fermented liquid prepared in Preparation Example 1 is centrifuged through a disc centrifuge, so that the fermented liquid is divided into an underflow (water content 90% by weight) rich in bacterial cells (the first bacterial cell ) and the fermentation raffinate top flow (the first fermentation raffinate), and the fermentation raffinate top flow enters the fermentation raffinate temporary storage tank.

[0109] (2) the underflow that is rich in the target thalline cell that step (1) obtains is washed 2 times with equal volume of water, then pumped into a belt vacuum filter and filtered, and the thalline (water content 75% by weight) after the filter is dried %) (the second bacterial cell) enters the ultrasonic-assisted extraction tank, and the filtrate (second fermentation raffinate) enters the fermentation raffinate temporary storage tank.

[0110] (3) pump the mixed solution of the fermentation raffinate and the filtrate in the temporary ...

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Abstract

The invention relates to the technical field of PHA extraction and separation, and discloses a method and system for extracting polyhydroxyalkanoate with ammonia water and ultrasound, wherein the method includes: (1) combining ammonia water with bacterial cells containing polyhydroxyalkanoate Mix to obtain the bacterial suspension and perform ultrasonic crushing to obtain a polyhydroxyalkanoate-containing slurry; (2) subject the obtained polyhydroxyalkanoate-containing slurry to a first solid-liquid separation to obtain the polyhydroxyalkanoate; The method and system of the present invention can effectively improve the yield and purity of polyhydroxyalkanoate, ensure the polyhydroxyalkanoate with higher degree of polymerization, and reduce the cost.

Description

technical field [0001] The invention relates to the field of extraction and separation of polyhydroxyalkanoate, in particular to a method and system for extracting polyhydroxyalkanoate with ammonia water and ultrasonic waves. Background technique [0002] Polyhydroxyalkanoate (PHA) is a general term for a class of polymer polyesters that are completely synthesized by microorganisms. PHA is biodegradable and biocompatible, so it is considered as an environmentally friendly material, which helps to solve the increasingly serious environmental pollution problem. [0003] Although the use of PHA can effectively avoid the harm caused by petrochemical plastics to the environment, at present, the production cost of this environmentally friendly bioplastic is high, and the recovery rate and purity of PHA extracted and separated by the existing technology are low. There are two directions for the extraction and separation of PHA. The first direction is to select organic reagents (su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G63/06
CPCC08G63/06
Inventor 佟毅李义郭元亨彭超陈博陶进杨凯武丽达
Owner COFCO NUTRITION & HEALTH RES INST
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