Application of perphenazine in preparing medicament for treating endometrial carcinoma
A technology of endometrial cancer and perphenazine, which is applied in the field of drug therapy to achieve the effects of inhibiting growth, inducing apoptosis, and inhibiting phosphorylation
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Embodiment 1
[0032] Proliferation inhibitory effect of perphenazine on endometrial cancer cells Ishikawa (ISK, type I, progesterone-sensitive), KLE cells (type II, progesterone-resistant), HEC-1A, HEC-1B and AR3CAN
[0033] 1. Experimental materials and methods
[0034] Endometrial cancer cells ISK, KLE, HEC-1A, HEC-1B and AR3CAN were purchased from the American Type Culture Collection (ATCC); phosphate buffered saline (PBS), DME / F12 medium were purchased from Hyclone; Serum (FBS) was purchased from Gibco; pancreatin and CCK8 were purchased from Beyontian Biotechnology Co., Ltd.; perphenazine (PPZ) and medroxyprogesterone acetate (MPA) were from the old drug library of the laboratory. The specific experimental method is as follows:
[0035] 1) Take the cells in the logarithmic growth phase, pour off the culture medium in the culture dish, and add 2 mL of PBS to wash the cells twice.
[0036] 2) Add 1 mL of trypsin and digest at room temperature for 2 min.
[0037] 3) Add 2 mL of culture...
Embodiment 2
[0049] Effects of perphenazine and MPA on the colony formation of Ishikawa and KLE cells
[0050] 1. Experimental materials and methods
[0051] Crystal violet was purchased from Biyuntian Biotechnology Co., Ltd.; methanol is a commonly used reagent in the laboratory, purchased commercially, without any treatment. All the other experimental materials are from the same sources as in Example 1. The specific experimental method is as follows:
[0052] 1) Take the cells in the logarithmic growth phase, digest them with trypsin to prepare a single-cell suspension, serially dilute, inoculate into a 6-well plate at a density of about 800 cells per well, and wait overnight for them to adhere to the wall.
[0053] 2) Five experimental groups were established, which were control group; MPA 20 μM group; MPA 30 μM group; PPZ 20 μM group; PPZ 30 μM group. The drug-administered group was cultured with 1 mL of DME / F12 medium containing different concentrations of drugs for 2 days, and the...
Embodiment 3
[0062] Effects of perphenazine and MPA on the migration ability of Ishikawa and KLE cells
[0063] 1. Experimental materials and methods
[0064] Paraformaldehyde is a commonly used reagent in the laboratory, commercially purchased without any treatment, and the sources of the rest of the experimental materials are the same as in Example 1. The specific experimental methods are as follows:
[0065] 1) Take 100 μL (about 200,000 cells) of serum-free ISK / KLE cell suspension and add it to the upper chamber of the Transwell chamber. The medium used for the cell suspension is serum-free DME / F12 medium.
[0066] 2) Add 600 μL of DME / F12 medium containing 20% FBS to the lower chamber, taking care to avoid the generation of air bubbles.
[0067] 3) Three experimental groups were established, which were control group; MPA 10 μM group; PPZ 10 μM group. 100 μL of serum-free DME / F12 medium containing different drugs was added to the upper chamber of the administration group. Add 100...
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