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Application of hsa - circRNA6448-14 in diagnosis and prognosis prediction of esophageal squamous cell carcinoma

A technology for esophageal squamous cell carcinoma and prognosis, applied in the field of medicine and biology, can solve the problems of unclear mechanism of action, different expression profiles of circRNAs, and lack of application exploration.

Inactive Publication Date: 2020-06-12
安阳市肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of circRNA in esophageal squamous cell carcinoma is still unclear, and the application of circRNA as a biomarker of ESCC is still lack of exploration
Moreover, due to the inconsistency of the detection platform, the circRNA expression profiles of ESCC are not the same

Method used

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  • Application of hsa - circRNA6448-14 in diagnosis and prognosis prediction of esophageal squamous cell carcinoma
  • Application of hsa - circRNA6448-14 in diagnosis and prognosis prediction of esophageal squamous cell carcinoma
  • Application of hsa - circRNA6448-14 in diagnosis and prognosis prediction of esophageal squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Construction of circRNA expression profiles, screening of differentially expressed circRNAs

[0038] 1. Sample source and sample processing

[0039] From June 4, 2015 to April 5, 2016, 6 patients who underwent radical resection of esophageal cancer in the Department of Thoracic Surgery of Anyang Cancer Hospital were selected and diagnosed as esophageal squamous cell carcinoma by histopathology. Among them, there were 3 males and 3 females, aged 45 to 77 years, with a median age of 61 years. All patients did not receive any radiotherapy, chemotherapy, targeted or other anti-tumor treatments before surgery. During the operation, ESCC tissues and paired paracancerous normal tissue samples were collected, and stored in a -80°C ultra-low temperature freezer for future use. The matched paracancerous normal tissue was taken from the edge of the esophageal cancer tissue more than 5cm away. There was no obvious abnormality in the naked eye, and it was confirmed as a...

Embodiment 2

[0081] Example 2: Screening out 5 differentially expressed circRNAs and identifying key circRNAs

[0082] According to the difference fold, p value and signal value, 5 circRNAs were screened from the 15908 significantly differentially expressed circRNAs selected in Example 1, namely: hsa_circRNA6448-14, hsa_circ_0110255, hsa_circRNA15930-8, hsa_circ_0064369 and hsa_circ_0024108,3 1 up-regulated and 2 down-regulated, all originating from exons. See Table 1 for detailed biological information. The expression of 5 circRNAs in 26 pairs of ESCC tissues and adjacent tissues was verified by the first step qRT-PCR experiment, and the key circRNAs were identified; The expression of key circRNAs in tissues was verified.

[0083] Table 1 Five differentially expressed circRNAs screened in ESCC

[0084] name P value FC value trend Chr source gene abbreviation hsa-circRNA6448-14 0.002686278 23.9508689 up chr5 exon TGFBI hsa_circ_0110255 7.52115E-...

Embodiment 3

[0123] Example 3: Application of hsa_circRNA6448-14 in prognosis prediction of esophageal squamous cell carcinoma

[0124] In order to clarify the clinical significance of hsa_circRNA6448-14 expression in esophageal squamous cell carcinoma, the clinical data statistics and postoperative follow-up statistics of 76 patient samples collected in Example 2 were further analyzed, and the expression of hsa_circRNA6448-14 in 76 patient samples was analyzed according to the statistical results Relationship with ESCC clinicopathological features and postoperative DFS (Disease-free Survival, disease-free survival period), OS (Overall Survival, overall survival period).

[0125] 1. Follow-up and statistical analysis

[0126] Cervical, chest and abdomen CT, color ultrasound of the upper abdomen and double neck every 3 months within 2 years after operation, and gastroscopy and PET-CT examination if necessary; re-examination every six months within 2 to 5 years; every 1 year after 5 years O...

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Abstract

The invention belongs to the technical field of medical biology, and particularly discloses a marker hsa - circRNA6448-14 for diagnosis and prognosis prediction of esophageal squamous cell carcinoma.The hsa - circRNA6448-14 is highly expressed in esophageal squamous cell carcinoma tissues, and is expressed in para-carcinoma normal tissues. By detecting the expression level of hsa - circRNA6448-14in tissues, the esophageal squamous cell carcinoma can be subjected to diagnosis and prognosis prediction. The invention provides a new approach for diagnosis and prognosis prediction of the esophageal squamous cell carcinoma, and provides a reference basis for clinicians to diagnose esophageal squamous cell carcinoma and analyze conditions of the esophageal squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and specifically relates to the application of hsa_circRNA6448-14 in the diagnosis and prognosis prediction of esophageal squamous cell carcinoma. Background technique [0002] Esophageal squamous cell carcinoma (ESCC), also known as esophageal squamous cell carcinoma, is a malignant tumor that occurs in human esophageal epithelial tissue. Cancer patients, more than half of which occur in China, the incidence rate is 100 times higher than that in Western countries. According to the latest national cancer report of the National Cancer Center in 2018, the number of new cases of esophageal cancer in my country in 2014 was 18.85 / 100,000, ranking sixth among malignant tumors, while the death rate of esophageal cancer was 14.11 / 100,000, ranking first among malignant tumors. fourth place. At present, the diagnosis and prognosis prediction of esophageal cancer face three major challenges: the late o...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 周福有张耀文樊锐太王立东杨海军李军扩代宁涛原翔
Owner 安阳市肿瘤医院
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