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Sugar alcohol in-vitro intestinal microorganism evaluation method

A technology of intestinal microbes and evaluation methods, applied in the field of in vitro intestinal microbe evaluation of sugar alcohols, can solve the problem of not being able to well reflect human metabolism and flora data, and unable to obtain data on the flora and its metabolites in the colon. It is difficult to get dynamic data changes and other issues

Inactive Publication Date: 2020-06-12
ZHEJIANG HUAKANG PHARMA
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Problems solved by technology

Existing animal experiments have differences in the composition of intestinal microbes and human intestinal microbes, so the experimental results cannot well reflect the metabolism and flora data of the human body
Although human experiments can understand the metabolism of the human body, the results only reflect the information of the rectum, and cannot obtain data on the colonic flora and their metabolites, and it is difficult to obtain dynamic data changes

Method used

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  • Sugar alcohol in-vitro intestinal microorganism evaluation method
  • Sugar alcohol in-vitro intestinal microorganism evaluation method
  • Sugar alcohol in-vitro intestinal microorganism evaluation method

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Embodiment 1

[0055] The embodiment of the first sugar alcohol intestinal microbial evaluation method in vitro of the present invention comprises the following steps:

[0056] Step 1. Prepare an in vitro mixed bacteria evaluation device. The structure of the in vitro mixed bacteria evaluation device is as described above, and will not be repeated here.

[0057] Step 2. Weigh 10g of fresh feces and fully stir and suspend them with 80mL, pH6.8 PBS buffer solution, then filter out solid particles with sterile three-layer gauze, and the obtained filtrate is the bacterial liquid containing fecal microorganisms, which is stored in an anaerobic environment spare.

[0058] Among them, fresh feces were collected by the following method. The feces of three healthy young people (20-25 years old) who had no intestinal diseases such as inflammatory bowel disease and irritable bowel syndrome and had not used antibiotics within half a year were collected as the source of intestinal microorganisms. After...

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Abstract

The invention relates to a sugar alcohol in-vitro intestinal microorganism evaluation method. Through an in-vitro mixed bacteria evaluation device, physical regulation and control, a pH and OD double-feedback system and mucous membrane globules are utilized to simulate a mucous layer of a human body, so that a more real colon environment can be simulated, and the quantity and variety composition of microorganisms have high similarity in the colon of the human body. A metabolism estimation model is realized by adopting single double-three-bacterium culture, and a plurality of more important florae in sugar alcohol metabolism are selected for single double-three-bacterium instead of simply selecting lactic acid bacteria or bifidobacteria for experiment by performing correlation analysis anda random number forest method on data obtained by an in-vitro simulated fermentation system CDMN model. Such a method enables more realistic and efficient data to be obtained. In addition, data of symbiotic nourishing of the intestinal microbial flora by utilizing sugar alcohol can be explored and obtained in double-three-bacterium culture, so that the defects of a single-bacterium experiment areovercome, and a more real metabolism estimation model is more favorably realized.

Description

technical field [0001] The invention belongs to the technical field of in vitro intestinal microbe evaluation of sugar alcohols, in particular to a method for in vitro intestinal microbiological evaluation of sugar alcohols. Background technique [0002] The existing research directions and technical routes on the utilization of sugar alcohols by microorganisms mainly involve the study of single bacteria such as lactic acid bacteria and bifidobacteria, that is, how sugar alcohols affect the metabolic activities of these single bacteria and the production of metabolites. However, in human digestion and in nature, the corresponding transformation and utilization of sugar alcohols is carried out in a complex microecology. For example, the utilization of sugar alcohols by microorganisms in the intestine is much more complicated than that of single bacteria. The reason is that intestinal microorganisms have a symbiotic nourishment effect in utilizing sugar alcohols and other preb...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/04C12M1/02C12M1/00C12Q1/04
CPCC12M23/02C12M23/58C12M27/02C12M29/06C12M33/04C12M33/12C12M41/12C12M41/26C12Q1/04
Inventor 朱炫向沙沙石丽华叶昆李勉
Owner ZHEJIANG HUAKANG PHARMA
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