A method for distinguishing Spartina and Spartina alterniflora

A technology of Spartina alterniflora and Spartina alterniflora, which is applied in the field of identifying Spartina alterniflora and Spartina alterniflora, and can solve problems such as high false positive rate, information loss, and overlapping peaks

Inactive Publication Date: 2021-07-27
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

However, due to the large genomes of Spartina alterniflora and Spartina alterniflora, which are hexaploid and dodecaploid respectively, and Spartina alterniflora is the maternal source of Spartina alterniflora, it is impossible to distinguish the two species using chloroplast gene fragments, while microsatellites, etc. Traditional molecular markers generally have problems such as poor consistency of experimental results, high false positive rate, and information loss in polyploid materials.
Moreover, due to the origin of hybridization, the first-generation sequencing results of Spartina ITS and partial nuclear gene sequences have serious overlapping peaks, and negative factors such as the inability to accurately determine the base of the site

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  • A method for distinguishing Spartina and Spartina alterniflora
  • A method for distinguishing Spartina and Spartina alterniflora

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Embodiment

[0029] The dried leaves of 16 individuals of Spartina alterniflora and Spartina alterniflora were selected, of which Spartina sativa was collected from Dandong City and Huludao City, Liaoning Province, and Spartina alterniflora was collected from Dongying City, Shandong Province, Chongming District, Shanghai, Yancheng City, Jiangsu Province, Taishan City, Guangdong Province (see Table 1 below for details).

[0030] Table 1. Sample Information Sheet

[0031] plant name Numbering collection place Longitude (°E) Dimensions (°N) Spartina (S. anglica) 1 Liaoning Dandong 124.209 39.872 Spartina (S. anglica) 2 Liaoning Dandong 124.209 39.872 Spartina (S. anglica) 3 Liaoning Dandong 124.209 39.872 Spartina (S. anglica) 4 Liaoning Dandong 124.209 39.872 Spartina (S. anglica) 5 Liaoning Huludao 120.962 40.799 Spartina (S. anglica) 6 Liaoning Huludao 120.962 40.799 Spartina (S. anglica) 7 Liaoning Huluda...

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Abstract

The invention provides a method for distinguishing Spartina and Spartina alterniflora, which comprises adding designed primers to the DNA of a sample to be tested and performing amplification. The method of the present invention develops a method for distinguishing molecular markers by stabilizing differential DNA fragments, and the identification and distinction of two species can be carried out only through simple PCR and agarose gel electrophoresis results, with low cost, simple operation and fast , Accurate and convenient for inspection and quarantine.

Description

technical field [0001] The invention relates to the identification and identification of alien invasive species, and specifically relates to a method for identifying Spartina spartina and Spartina alterniflora. Background technique [0002] Biological invasion has become one of the most important factors threatening the protection of global biodiversity. With the development of my country's global trade and tourism, my country's vast territory and complex and diverse natural environment have provided a superior living space for all kinds of alien organisms. , has become one of the countries most severely affected by alien biological invasion in the world. [0003] Spartina alterniflora and S. anglica are two grasses of the genus Spartina. Spartina alterniflora is produced in North America and is a typical invasive alien plant in coastal tidal flat wetlands in my country. It is also one of the alien species included in the "First List of Invasive Alien Species in China". Aft...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 李飞飞赵彩云高珂晓柳晓燕朱金方
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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