Method for identifying spartina anglica and spartina alterniflora

Abstract

The present invention provides a method for identifying spartina anglica and spartina alterniflora. The method comprises adding a designed primer to DNA of a sample to be tested and conducting amplification. The method develops a molecular marker discrimination method by stably different DNA fragments, the two species can be identified and distinguished by simple PCR and agarose gel electrophoresis results, and the method is low in cost, simple in operation, rapid and accurate, and brings convenience to inspection and quarantine.

Description

technical field [0001] The invention relates to identification and identification of alien invasive species, and specifically relates to a method for identifying Spartina alterniflora and Spartina alterniflora. Background technique [0002] Biological invasion has become one of the most important factors threatening the protection of global biodiversity. With the development of my country's global trade and tourism, my country's vast territory and complex and diverse natural environment provide a superior living space for various alien organisms , has become one of the countries most severely invaded by alien organisms in the world. [0003] Spartina alterniflora (Spartina alterniflora) and ricegrass (S. anglica) are two species of Spartina in the Poaceae family. Native to North America, it is a typical invasive alien plant in coastal wetlands in my country, and it is also one of the alien species included in the "First Batch of Invasive Alien Species List in China". After ...

AI Technical Summary

Problems solved by technology

However, due to the large genomes of Spartina alterniflora and Spartina alterniflora, which are hexaploid and dodecaploid respectively, and Spartina alterniflora is the maternal source of Spartina alterniflora, it is impossible to distinguish the two species using chloroplast gene fragments, while microsatellites, etc. Traditional molecular markers generally have problems such as poor consistency of experimental results, high false positive rate, and information loss in polyploid materials.

Moreover, due to the origin of hybridization, the first-generation sequencing results of Spartina ITS and partial nuclear gene sequences have serious overlapping peaks, and negative factors such as the inability to accurately determine the base of the site

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