Specific PCR primers used for detecting mycoplasma capricolum subsp. capripneumoniae

Abstract

The invention discloses specific PCR primers used for detecting mycoplasma capricolum subsp. capripneumoniae. According to the specific PCR primers used for detecting the mycoplasma capricolum subsp.capripneumoniae, firstly, by conducting gene sequencing and comparing on the mycoplasma capricolum subsp. capripneumoniae, a target sequence used for detecting the mycoplasma capricolum subsp. capripneumoniae is screened out, a gene sequence of the target sequence is shown in SEQ ID NO.1 as shown in the description, then the specific PCR primers used for detecting the mycoplasma capricolum subsp.capripneumoniae are designed, the specific PCR primers used for detecting the mycoplasma capricolum subsp. capripneumoniae can distinguish the Mccp (mycoplasma capricolum subsp. capripneumoniae) fromother members of mycoplasma capricolum clusters and common ruminant mycoplasmas, show good specificity, and can be used as a novel diagnosis target, and on the basis of the specific PCR primers used for detecting the mycoplasma capricolum subsp. capripneumoniae, an Mccp diagnosis technology with higher clinical practicability is built.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a specific PCR primer for detecting mycoplasma goat pneumonia subspecies. Background technique [0002] Contagious caprine pleuropneumonia (CCPP) is a highly contagious goat respiratory disease caused by Mycoplasma capricolumsubsp. The rate is 60% to 80%, and it is one of the OIE legally notifiable diseases. The goat industry in more than 40 countries around the world is threatened by CCPP. Our country first reported goat "rotten lung disease" in Xinjiang in 1922, and it was widely popular in Northwest and North China. It is easy to control, but in recent years, the number of case reports has increased, showing a comeback trend, and the difficulty of control has increased. [0003] The CCPP pathogen Mccp belongs to the mycoplasma cluster. In addition to Mccp, members of this cluster include: Mycoides subsp.mycoides (Mmm), Mycoides subsp.capri (M.mycoides s...

AI Technical Summary

Problems solved by technology

With the continuous improvement of technology, these nucleic acid diagnostic methods have good specificity and sensitivity, but on the one hand, most of the amplification systems are based on DNA samples, which require professional laboratory equipment; and there is no need to extract DNA and professional experiments The RPA technology detection cost of the equipment is as high as 80 yuan / sample, which greatly reduces the clinical practicability of nucleic acid diagnostic methods for detecting Mccp

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