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Ultrasonic wave synergetic biological enzyme preparation method for nelumbo nucifera gaertn resistant starch

A technology of resistant starch and ultrasound is applied in the field of deep processing of lotus root to achieve the effect of increasing added value and good food processing characteristics

Inactive Publication Date: 2020-06-05
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on simultaneous starch hydrolysis by ultrasound and enzymatic method

Method used

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  • Ultrasonic wave synergetic biological enzyme preparation method for nelumbo nucifera gaertn resistant starch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: The effect of the amount of pullulanase added on the preparation of lotus root resistant starch by pullulan enzyme method

[0036] (1) Accurately weigh 2 g of lotus root starch into a 100 ml Erlenmeyer flask to obtain a starch suspension (4%, m / m), and place it in an autoclave for high temperature treatment at 121° C. for 20 min.

[0037] (2) Place it in a constant temperature water bath at 50°C, adjust the pH value with 0.05mol / L HCl solution, add pullulanase, and perform debranching treatment for 20 hours under the condition of a water bath at 50°C, with an addition amount of 9 npun / g (starch) .

[0038] (3) Cool to room temperature, then pour the sample into a petri dish, age at 4°C for 24 hours, freeze-dry for 48 hours, grind, package, and store in a desiccator. According to the national standard NY-T 2638-2014, the content of resistant starch in lotus root was determined to be 11.21%.

[0039] It shows that the amount of pullulanase added is 9 npun / g (...

Embodiment 2

[0040] Example 2: The effect of the amount of pullulanase added on the preparation of lotus root resistant starch by pullulan enzyme method

[0041] (1) Accurately weigh 2 g of lotus root starch into a 100 ml Erlenmeyer flask to obtain a starch suspension (4%, m / m), and place it in an autoclave for high temperature treatment at 121° C. for 20 min.

[0042] (2) Place it in a constant temperature water bath at 50°C, adjust the pH value with 0.05mol / L HCl solution, add pullulanase, and perform debranching treatment for 20 hours under the condition of a water bath at 50°C, with an addition amount of 9 npun / g (starch) and 45npun / g (starch).

[0043] (3) Cool to room temperature, then pour the sample into a petri dish, age at 4°C for 24 hours, freeze-dry for 48 hours, grind, package, and store in a desiccator. According to the national standard NY-T 2638-2014, the content of resistant starch in lotus root was determined to be 17.01%.

[0044] When the amount of pullulanase was 45n...

Embodiment 3

[0045] Example 3: Effect of Enzymolysis Time on Preparation of Lotus Root Resistant Starch by Pullulan Enzyme Method

[0046] (1) Accurately weigh 2 g of lotus root starch into a 100 ml Erlenmeyer flask to obtain a starch suspension (4%, m / m), and place it in an autoclave for high temperature treatment at 121° C. for 20 min.

[0047] (2) Place it in a constant temperature water bath at 50°C, adjust the pH value with 0.05mol / L HCl solution, add 27 npun / g (starch) of pullulanase, perform debranching treatment under the condition of 50°C water bath, enzymolysis Time 6h.

[0048] (3) Cool to room temperature, then pour the sample into a petri dish, age at 4°C for 24 hours, freeze-dry for 48 hours, grind, package, and store in a desiccator. According to the national standard NY-T 2638-2014, the content of resistant starch in lotus root was determined to be 11.51%. Compared with lotus root starch, there is basically no significant difference.

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Abstract

The invention discloses an ultrasonic wave synergetic biological enzyme preparation method for a nelumbo nucifera gaertn resistant starch, and belongs to the technical field of nelumbo nucifera gaertndeep processing. The preparation method is carried out according to the following steps of: (1) cleaning and tidying nelumbo nucifera gaertn; (2) extracting the nelumbo nucifera gaertn starch; (3) preparing the resistant starch; and (4) carrying out enzymolysis under an ultrasonic field, freezing, and drying to obtain the nelumbo nucifera gaertn resistant starch. Through a cavatition function anda mechanical vibration function of an ultrasonic wave, the movement of solvent molecules is quickened, and an extraction rate of a target product is improved. In a preparation process that the nelumbo nucifera gaertn resistant starch is prepared by a pullulanase method, a multi-mode ultrasonic wave treatment technology is used. Pullulanase is accelerated to realize enzymolysis by ultrasound in order to solve the problems of low enzymolysis reaction efficiency and the like in the process that the resistant starch is prepared by an enzymic method. The preparation method takes the nelumbo nucifera gaertn starch as a raw material to prepare the nelumbo nucifera gaertn resistant starch. Since the resistant starch has various physiological functions and good food processing characteristics, thepreparation method disclosed by the invention is favorable for the comprehensive utilization of the nelumbo nucifera gaertn, and the additional value of the nelumbo nucifera gaertn is improved.

Description

technical field [0001] The invention relates to the technical field of lotus root deep processing, in particular to a method for preparing lotus root resistant starch by using lotus root as a raw material and adopting ultrasonic and biological enzymatic treatment. Background technique [0002] Lotus root (Nelumbo nucifera gaertn), also known as lotus root, referred to as lotus root, is a perennial plant of Nymphaeaceae. It is mainly distributed in the three major river basins of the Yangtze River, the Yellow River and the Pearl River in China. excellent. Lotus root is rich in starch, protein, vitamins, iron, calcium, alkaloids and other substances that are beneficial to human health. It has high edible and medicinal value, and is an aquatic economic crop with a wide range of uses. According to "Compendium of Materia Medica" records: lotus root, born sweet and cold, can cool blood and stop bleeding, remove heat and clear stomach, so it mainly dissipates congestion, relieves ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/16
CPCC12P19/04C12P19/16
Inventor 任晓锋汤佳琳刘宇轩陈薪乡汪在虎梁秋芳孙璐邢梦德曹学润段兴艳
Owner JIANGSU UNIV
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