The lcye mutant of the key gene of wheat carotenoid synthesis pathway and its application

Abstract

The invention discloses a mutant of the key gene Lcye in the synthetic pathway of wheat carotenoid and its application. The present invention provides the following protein: the protein obtained by replacing the serine at position 253 of the Lcye-D1 protein with phenylalanine, or by substituting and / or deleting and / or adding one or several amino acid residues and a derivative protein with the same ability, or a protein with more than 99%, more than 95%, more than 90%, more than 85% or more than 80% homology and the same function with its defined amino acid sequence, or at its N-terminal And / or the fusion protein obtained after the C-terminus is linked with a tag. The invention not only verifies the function of the Lcye gene, but also provides a theoretical basis and germplasm resources for improving the color properties of flour and its products.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mutant of the key gene Lcye in the synthesis pathway of wheat carotenoids and an application thereof. Background technique [0002] Flour color is an important sensory index and market index to evaluate the quality of wheat flour. Yellow pigment is the most important natural pigment in wheat grain, and it is the main reason for the yellowness of flour and its products. The correlation coefficients between grain yellow pigment content and flour and dough yellowness are as high as 0.8-0.9, and the correlation coefficients with bread and noodle color are 0.69 and 0.76 (Mares and Campbell, 2001; Adom et al., 2003; Fratianni et al., 2005; Cong et al., 2010). Chinese noodles, such as white noodles, steamed buns, steamed buns and dumplings, have relatively high requirements on the whiteness of flour, and fine and white flour and its products are very popular. [0003] Carotenoids are t...

AI Technical Summary

Problems solved by technology

In summary, the current research on wheat Lcye gene is limited to QTL mapping, gene cloning and molecular marker development, and its gene function and genetic regulation mechanism are still unclear, which seriously restricts the progress of breeding work.

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