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Artificial feeding method of diaphorina citri kuwayama

A citrus psyllid and artificial technology, applied in the field of agricultural science, can solve the problem of low trapping and killing rate of citrus psyllid, and achieve the effects of simple and easy artificial feeding method, low feeding cost and simple feeding device

Inactive Publication Date: 2020-05-12
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many successful examples of biological control of citrus psyllids, and the trapping and killing rate of citrus psyllids by yellow boards is low. In this case, the development of citrus psyllid sex pheromones is particularly urgent

Method used

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  • Artificial feeding method of diaphorina citri kuwayama
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  • Artificial feeding method of diaphorina citri kuwayama

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Embodiment

[0023] 1) Make a feeding device:

[0024] Take a transparent small plastic cup, the bottom diameter of the small plastic cup is 4.5cm, the opening diameter is 6.5cm, and the height is 8.5cm, and the bottom is provided with drainage holes. The schematic diagram of its structure is as follows: figure 1 shown. Nutritious soil is housed in the small plastic cup, and the nutritive soil will be sterilized by high temperature before use, and the thickness of the nutrient soil in the small plastic cup is 6cm. Buy Jiulixiang seedlings in the flower market, transplant single seedlings into plastic cups, and cultivate them with nutrient soil. After successful planting, trim them so that the height of the nutrient soil exposed is 8-10cm. The structure of Jiulixiang cultivation Schematic such as image 3 shown. Keep the temperature at 28±0.5°C, relative humidity at 60±5%, and photoperiod at 14L:10D. After 5 to 7 days, the Gourilla japonica seedlings will germinate and grow tender shoots...

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Abstract

The invention belongs to the field of agricultural science and in particular relates to an artificial feeding method of diaphorina citri kuwayama. The method comprises the following steps: putting nutrient soil into a small transparent plastic cup, and culturing a diaphorina citri kuwayama host plant, wherein drainage holes are formed in the bottom of the small plastic cup; inoculating diaphorinacitri kuwayama with the host plant, covering the small plastic cup by using a large transparent plastic cup in an embedding manner, and performing culture under conditions that the temperature is keptto 28+ / -0.5 DEG C, the relative humidity is 60+ / -5% and the light cycle is 14L:10D, so as to obtain imagoes of diaphorina citri kuwayama, wherein vent holes are formed in the top of the covering large plastic cup. The artificial feeding method of the diaphorina citri kuwayama, which is provided by the invention, is simple and feasible, and is particularly applicable to indoor breeding, and the life history of the diaphorina citri kuwayama can be observed. By adopting the method, an insect source material can be provided for research and development of sex pheromone, screening of medicines, resistance monitoring and resistance risk evaluation on the diaphorina citri kuwayama, meanwhile, a breeding device is simple, the breeding cost is low, breeding host plants and the like can be repeatedly used for multiple times, and the cost can be reduced.

Description

technical field [0001] The invention belongs to the field of agricultural science, and in particular relates to an artificial breeding method for citrus psyllids. Background technique [0002] The citrus psyllid Diaphorina citri Kuwayama belongs to the Hemiptera Psyllidaceae family, which is harmful to Rutaceae plants such as citrus, orange, pomelo, moon orange, citron, yellow bark, and currant. The devastating disease, Huanglongbing, the main vector of the Asian species "Candidatus Liberibacter asiaticus" and the American species "CaL.americanus", poses a serious threat to citrus production worldwide. Adults of citrus psyllid scatter and suck on leaves and young shoots, and nymphs cluster on new shoots, shoots and young leaves, sucking juice from shoots, and the damaged shoots and shoots shrivel and dry, and new leaves are deformed and easy to fall off. It affects the growth of plants, and the white secretion secreted by nymphs spreads on the branches and leaves, affects p...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G17/00
CPCA01G17/005A01K67/033
Inventor 郑丽霞梁琪椿陈文胜
Owner FOSHAN UNIVERSITY
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