Veterinary product
A technology of animal mites and allergens, applied in purified animal allergen extracts, more especially dog allergies, treatment of mammals, house dust mite allergies, and can solve sensitivity and other problems
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[0085] The source material (which may also be referred to as starting material) for the production of the extract is a mite culture with >80% D. farinae bodies grown by Laboratorios LETI and is commercially available. The three animal extracts were prepared according to the following methods:
[0086] A. Extract I
[0087] ●Weigh the raw material to be extracted and record the weight;
[0088] ● Calculate the volume of extractant (PBS 0.01M-NaCl 0.15M) required using the following formula:
[0089] Raw material (g) x 20=ml extractant;
[0090] - Place the raw material in a container containing half the extractant volume (calculated in the previous step) and extract for 4 hours at 3°C-5°C with magnetic stirring:
[0091] ml extractant (total volume) ÷ 2=ml extractant (volume 1);
[0092] Centrifuge the mixture at 10,000 rpm for at least 30 minutes at 5°C;
[0093] - Then recover the supernatant and record the volume. The supernatant was kept at 3°C-5°C in a labeled airt...
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