Primer and probe for rapidly detecting and identifying cryptococcus, and applications thereof

A technology for Cryptococcus and Cryptococcus neoformans, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of cumbersome operation, high error rate, low sensitivity, etc., and achieve simple operation, strong applicability, Sensitive effect

Active Publication Date: 2020-04-14
北京量觉科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of tedious operation, low sensitivity and high error rate of the traditional method for detecting Cryptococcus neoformans and Cryptococcus gattii, the present invention provides a primer and probe for rapid detection and identification of Cryptococcus and its application

Method used

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  • Primer and probe for rapidly detecting and identifying cryptococcus, and applications thereof
  • Primer and probe for rapidly detecting and identifying cryptococcus, and applications thereof
  • Primer and probe for rapidly detecting and identifying cryptococcus, and applications thereof

Examples

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Embodiment 1

[0027] Target genes, primers, probes and kits for detection and identification of Cryptococcus neoformans and Cryptococcus gattii

[0028] Dozens of genome sequences of Cryptococcus neoformans and Cryptococcus gattii were obtained through high-throughput sequencing technology, and the genome sequence differences of dozens of Cryptococcus were compared by genomics methods, and CAP59 was finally selected through preliminary primer and probe design The sequence is used as the target gene, and the gene fragment is relatively conserved in the two Cryptococcus species, and there are certain differences between Cryptococcus neoformans and Cryptococcus gattii. The nucleotide sequences of target genes for detecting Cryptococcus neoformans and Cryptococcus gattii are shown in SEQ ID NO:5 and SEQ ID NO:6.

[0029] Design primers for the selected target gene sequence information. Through repeated comparative analysis, real-time fluorescent PCR detection primers and probes that can be use...

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Abstract

The invention belongs to the field of fungal etiology detection, and discloses a primer and a probe for detecting cryptococcus, wherein the primer is a universal primer for detecting cryptococcus neoformans and cryptococcus gattii, and the probe comprises a probe for detecting cryptococcus neoformans and cryptococcus gattii. According to the invention, the raw materials required for detecting cryptococcus neoformans and cryptococcus gattii are put into the same reaction system, so that cryptococcus neoformans and cryptococcus gattii can be detected and identified at the same time, the varietyof pathogenic bacteria is clear, rapid and accurate clinical diagnosis is facilitated, and precious time is gained for disease treatment.

Description

technical field [0001] The invention belongs to the field of fungal etiology detection, in particular to primers and probes for rapid detection and identification of cryptococcus and applications thereof. Background technique [0002] In fungal taxonomy, Cryptococcus belongs to Deuteromycotina, Bacillus, Cryptococcus, and Cryptococcus. There are many species in Cryptococcus, which are widely distributed in nature. Cryptococcus neoformans and Cryptococcus gattii. In recent years, cryptococcal infection is on the rise at home and abroad. [0003] Because cryptococcal infection is easily misdiagnosed based on clinical manifestations, etiological detection is the main basis for diagnosis and treatment. At present, the main cryptococcus detection methods have limitations. Among them, cerebrospinal fluid ink staining can initially observe the morphology of cryptococcus, but the sensitivity is low; Cryptococcus special; Cryptococcus culture identification is also difficult to id...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/04C12N15/11C12R1/645
CPCC12Q1/686C12Q1/6895C12Q2563/107
Inventor 不公告发明人
Owner 北京量觉科技有限责任公司
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