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Hormone kit for scatophagus argus spawning induction and artificial spawning induction and insemination method for obtaining scatophagus argus fertilized eggs in batches

A technology of artificial induced spawning and money fish, which is applied in application, fish farming, pharmaceutical formula, etc., can solve the problems of low fertilization rate and hatching rate, can not meet the needs of large-scale production, high success rate of induced spawning, etc., and achieve the solution effect time long effect

Active Publication Date: 2020-04-14
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, luteinizing hormone-releasing hormone analogs (LHRH-A), luteinizing hormone-releasing hormone analogs (LHRH-A2), 17α, 20β-dihydroxyprogesterone + human chorionic gonadotropin (HCG) Melanin (MT) and other hormones are used to artificially induce spawning of broodfish. Some methods have not achieved practical results, and some methods can spawn naturally. However, due to the influence of the environment, the success rate of induced spawning, fertilization rate and hatching rate are very low, and it is difficult to obtain in batches. Fertilized eggs cannot meet the needs of large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Selection of induced broodstock

[0047] Select 2-year-old, healthy fish, and mature-promoted goldfish as broodstock to be induced. The gonads of the female fish have developed to stage IV, and the abdomen is full and slightly swollen. The male fish lightly presses the abdomen near the genital hole, and white semen flows out. The number of males and females The ratio is 1:2, and the male and female broodstock are placed in the waiting pond respectively.

[0048] 2. Three-needle artificial induction of labor

[0049] The first hormone injection: intraperitoneal injection at the depression at the base of the pectoral fin, the drug and dosage for female fish are 3 μg of gonadotropin-releasing hormone A4 (GnRH-A4) + 3 mg of dioconone maleate (DOM) per 1 kg body weight, and 3 mg for male fish Without injection, the male and female broodstock are placed in 500L hatching buckets for temporary breeding for later observation and operation;

[0050] The second hormone inject...

Embodiment 2

[0059] 1. Selection of induced broodstock

[0060] Select 2-year-old or older, healthy fish, which have been bred to promote maturation, as broodstock to be induced. The gonads of female fish have developed to stage IV, and the abdomen is full and slightly swollen. When the male fish lightly presses the abdomen, white semen flows out near the genital opening. The ratio of male to female The ratio is 1:2, and the male and female broodstock are placed in the waiting pond respectively.

[0061] 2. Three-needle artificial induction of labor

[0062] The first hormone injection: intraperitoneal injection at the depression at the base of the pectoral fin, the drug and dosage for female fish are injected with gonadotropin-releasing hormone A4 (GnRH-A4) 5 μg + dioconone maleate (DOM) 3 mg per 1 kg body weight, male fish Without injection, the male and female broodstock are placed in 500L hatching buckets for temporary breeding for later observation and operation;

[0063] The second...

Embodiment 3

[0072] 1. Selection of induced broodstock

[0073] Select 2-year-old or older, healthy fish, which have been bred to promote maturation, as broodstock to be induced. The gonads of female fish have developed to stage IV, and the abdomen is full and slightly swollen. When the male fish lightly presses the abdomen, white semen flows out near the genital opening. The ratio of male to female The ratio is 1:3, and the male and female broodstock are placed in the waiting pond respectively.

[0074] 2. Three-needle artificial induction of labor

[0075] The first hormone injection: intraperitoneal injection at the depression at the base of the pectoral fin, female fish injection drug and dosage: gonadotropin-releasing hormone A4 (GnRH-A4) 5 μg + dioconone maleate (DOM) 5 mg per 1 kg body weight, male fish Without injection, the male and female broodstock are placed in 500L hatching buckets for temporary breeding for later observation and operation;

[0076] The second hormone inject...

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Abstract

The invention provides a hormone kit for scatophagus argus spawning induction and an artificial spawning induction and insemination method for obtaining scatophagus argus fertilized eggs in batches, which belong to the technical field of aquaculture. The hormone kit for scatophagus argus spawning induction comprises gonadotropin releasing element A4, doxolone maleate and human chorionic gonadotropin which are independently subpackaged. Based on the hormone kit, scatophagus argus is subjected to artificial spawning induction by adopting a three-needle method, so that the problems of long effecttime, female scatophagus argus stagnation, high difficulty in artificial spawn extrusion after spawning induction, egg mass and gonad degeneration are effectively solved. The artificial inseminationmethod is adopted, and the problems that natural spawning eggs of scatophagus argus are over-mature or collapse in vivo, the influence of the environment is large, the spawning induction success rate,the fertilization rate and the hatching rate are extremely low, and fertilized eggs are difficult to obtain in batches are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a hormone kit for inducing labor in goldfish and a method for artificially inducing labor and insemination for obtaining batches of fertilized eggs in goldfish. Background technique [0002] Scatophagus argus Linnaeus (1766) belongs to Perciformes, Acanthuroidei, Scatophagidae, Scatophagus, widely distributed in the Indo-Pacific waters, mainly in China From the southern part of the East China Sea to the South China Sea and Beibu Gulf. The money fish has a beautiful body, colorful colors, gentle temperament and delicious meat. It is a rare seawater economic fish with ornamental and edible value. Money fish has strong environmental adaptability and disease resistance. It can not only grow and develop in seawater and brackish water, but also is suitable for freshwater cultivation. It has become an important species in ponds and cages in southern my country in recent...

Claims

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Application Information

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IPC IPC(8): A61K38/24A61K31/454A61P15/08A01K61/10
CPCA61K38/24A61K31/454A61P15/08A01K61/10A61K2300/00Y02A40/81
Inventor 黄洋李广丽朱春华江东能陈华谱石红娟田昌绪邓思平吴天利
Owner GUANGDONG OCEAN UNIVERSITY
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