Method for studying the mechanism of anti-insomnia effect of Sugemule based on amino acid metabolomics

A technology of metabolomics and mechanism of action, which is applied in the field of amino acid metabolomics to study the mechanism of anti-insomnia effect of Sugemule, which can solve the problems of low polarity and volatility, chromatographic column adsorption, etc.

Active Publication Date: 2021-12-17
田继兰
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Problems solved by technology

Metabolites in living organisms, such as amino acids, fatty acids, amines, sugars, and nucleotides, have the common characteristics of strong polarity and low volatility, and are easily adsorbed by chromatographic columns, so they are not suitable for direct GC-MS analysis

Method used

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  • Method for studying the mechanism of anti-insomnia effect of Sugemule based on amino acid metabolomics
  • Method for studying the mechanism of anti-insomnia effect of Sugemule based on amino acid metabolomics
  • Method for studying the mechanism of anti-insomnia effect of Sugemule based on amino acid metabolomics

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Embodiment Construction

[0039] The present invention will be further described with reference to the drawings and embodiments.

[0040] In one embodiment, as figure 1 As shown, the method for studying the mechanism of anti-insomnia of Sugemule based on amino acid metabolomics:

[0041] 1. Making PCPA to insomnia rat model

[0042] Rats were randomly divided into normal control group, model control group, Mongolian medicine group and Anshenbunao oral liquid group according to body weight, with 10 rats in each group; except for the normal control group, the rats in the other 3 groups were treated in the abdominal cavity at 8:00 every morning. Inject PCPA suspension 1 ml / 100g, once a day, for 2 consecutive days, and the normal control group was injected intraperitoneally with the same volume of weakly alkaline saline; 24-28 hours after the first intraperitoneal injection, the animal's circadian rhythm disappeared During the day, they were also active during the day, indicating that the model was succe...

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Abstract

The method of studying the anti-insomnia mechanism of Sugemule based on amino acid metabolomics is to use GC-MS method to analyze the amino acid metabolome in the brain tissues of PCPA insomnia model rats before and after Sugemule gavage. Source compounds, using PCA and PLS-DA modes to analyze changes in metabolite profiles in various parts of the brain and identify related metabolic markers; use LC-MS to carry out targeted metabolomics research, and investigate 5-Hydroxytryptamine, GABA in brain tissue , Glu and other neurotransmitters and related metabolite content changes; by looking for differential metabolites and their metabolic pathways related to the curative effect of Sugemule in clinical rat brain tissue samples, the mechanism and location of the anti-insomnia effect of Sugemule were clarified point; the present invention applies metabolomics research means for the first time, and preliminarily proves the mechanism of action of Sugemule regulating sleep-wake rhythm through the neurotransmitter system.

Description

technical field [0001] The invention belongs to the cross technical field of traditional Chinese medicine technology and metabolomics technology, specifically a method for studying the mechanism of Sugemule's anti-insomnia action based on amino acid metabolomics. Background technique [0002] Sleep accounts for about one-third of a normal person's daily time, and is an important physiological process to maintain normal life activities of the human body. In recent years, with the rapid development of the economy, the pace of life is accelerating, the pressure of occupation and environment is increasing, the incidence of insomnia is also increasing, and the trend of younger people is becoming more and more obvious, which seriously affects people's normal life. work, life and health. Insomnia has become a common and frequently-occurring disease in neurology outpatient clinics, with clinical manifestations such as difficulty in falling asleep, easy waking, dreaminess, early awa...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A61K49/00A61K36/9064A61P25/20
CPCA01K67/02A61K49/0008A61K36/9064A61K36/28A61K36/67A61K36/725A61P25/20A61K2300/00
Inventor 田继兰
Owner 田继兰
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