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Application of crude broussonetia papyrifera leaf extract in preparation of drugs used for treating non-alcoholic fatty liver disease

A technology for non-alcoholic and fatty liver disease, applied in drug combinations, metabolic diseases, pharmaceutical formulations, etc., can solve problems such as the unknown therapeutic effect of NAFLD

Inactive Publication Date: 2020-04-10
JILIN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, its therapeutic effect on NAFLD remains unknown

Method used

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  • Application of crude broussonetia papyrifera leaf extract in preparation of drugs used for treating non-alcoholic fatty liver disease
  • Application of crude broussonetia papyrifera leaf extract in preparation of drugs used for treating non-alcoholic fatty liver disease
  • Application of crude broussonetia papyrifera leaf extract in preparation of drugs used for treating non-alcoholic fatty liver disease

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Embodiment Construction

[0013] Further illustrate the present invention by the following examples, do not limit the present invention in any way, under the premise of not departing from the technical solution of the present invention, any modification or change that those of ordinary skill in the art that the present invention is done to realize easily will all be fall within the scope of the claims of the present invention.

[0014] 1. Extraction of Cone leaves

[0015] Grind the leaves into dry powder, heat them with 75% ethanol solution for 1 hour to reflux for extraction, the material ratio is 1:20, repeat the above operation, collect the extract twice, concentrate with a rotary evaporator, and dry at 70°C to obtain crude extracts of the leaves. things.

[0016] 2. Cell oil red staining experiment

[0017] HepG2 cells were cultured in DMEM medium containing 10% FBS, 100U / mL penicillin and 100U / mL streptomycin, and incubated in a constant temperature incubator at 5% CO2 at 37°C. Will be about 2...

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Abstract

The invention provides an application for a crude broussonetia papyrifera leaf extract in preparation of drugs used for treating the non-alcoholic fatty liver disease. Through measurement of the contents of triglyceride and total cholesterol in cell oil red O staining, mouse liver oil red O staining and mouse blood, the crude broussonetia papyrifera leaf extract is proved to be able to effectivelyinhibit aggregation of lipid in HepG2 cells, improve lipid accumulation in the liver and reduce the level of the cholesterol and the triglyceride in the mouse blood. According to the invention, in atest of treating the non-alcoholic fatty liver disease with the crude broussonetia papyrifera leaf extract, no obvious toxic effect is shown; meanwhile, the crude broussonetia papyrifera leaf extracthas good curative effect. Thus, the crude broussonetia papyrifera leaf extract can be used for development of new drugs, and has important significance on drug target confirmation.

Description

technical field [0001] The invention relates to the application of the crude extract of the leaves of sarcorrhizae in the preparation of medicines for treating non-alcoholic fatty liver disease, and belongs to the field of medicine. Background technique [0002] Nonalcoholic fatty liver disease (NAFLD) refers to a clinicopathological syndrome characterized by excessive fat deposition in liver cells caused by other liver damage factors except alcohol. It is a disease related to insulin resistance and genetic susceptibility Closely related to acquired metabolic stress-induced liver injury. During the disease process, a large amount of lipid accumulation and inflammatory response will occur, but there are often no obvious clinical symptoms, so patients are only discovered in the period of liver cirrhosis and severe hepatitis. The global population prevalence of NAFLD has been reported to be approximately 20%, and due to the prevalence of Western dietary habits in Asian countri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/60A61P1/16A61P3/06A61K127/00
CPCA61K36/60A61P1/16A61P3/06
Inventor 冯海华王齐杜润宝金美玉王建锋
Owner JILIN UNIV
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