Solid-state fermentation wheat bran feed, and preparation method and application thereof
A technology of solid-state fermentation and wheat bran, which is applied in the direction of biochemical equipment and methods, applications, animal feed, etc., can solve the problems of ineffective degradation of wheat bran, limited application, and poor cellulose decomposition effect, so as to improve the application value and reduce the Diarrhea rate, effect of increasing content
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Embodiment 1
[0039] Screening of Fermentation Strain Combinations Optimum for Cellulose Degradation
[0040] The present invention selects 3 kinds of bacterial strains (Aspergillus niger (H), Trichoderma viride (L), Neurospora crassa (C)) altogether. There are 7 combinations, they are H, L, C, HL, HC, LC, HLC, each combination is inoculated in solid medium according to the orthogonal design table to ferment wheat bran, the inoculation amount, material-water ratio, fermentation Time and fermentation temperature are the fermentation factors, and each factor is set to 3 levels, using L 9 ( 3 4) Orthogonal table (Table 2) An orthogonal fermentation experiment with four factors and three levels was carried out, with 9 groups in total, and each group had 3 repetitions. Orthogonal experimental design is shown in Table 1.
[0041] Table 1 Orthogonal experimental design
[0042]
[0043] Note: A represents the inoculation amount (0%), B represents the material-water ratio (W / V), C represents...
Embodiment 2
[0061] Screening of the Combination of Fermentation Strains Optimum for Producing Somatic Protein
[0062] The above-mentioned combinations with the top three cellulase activities were used to re-ferment the wheat bran according to the test conditions of Example 1, and immediately after the fermentation was completed, the Candida tropicalis suspension was added and mixed, and at the same time, it was fermented according to the same test conditions 1d, the combination with the highest crude protein content and the lowest crude fiber content in the obtained fermentation product is the optimum fermentation combination.
[0063] The changes in the amount of yeast suspension added, crude protein, crude fiber, etc. are shown in Table 7.
[0064] Table 7 Changes of crude protein and crude fiber content before and after solid-state fermentation of wheat bran
[0065]
[0066] Note: "-" means not added or none.
[0067] It can be seen from Table 7 that compared with unfermented wh...
Embodiment 3
[0075] After selecting the strain combination (HLC6+J), the present embodiment provides a solid-state fermented wheat bran feed, which consists of: 1 part of wheat bran, 1.2 parts of nutrient solution, Aspergillus niger-Trichoderma viride-rough 0.15 part of compound bacterial culture solution of Neurospora, 0.05 part of Candida tropicalis suspension, wherein, the composition of described nutrient solution is: every 1L distilled water contains (NH 4 ) 2 SO 4 5g, KH 2 PO 4 1g, CaCl 2 0.6g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.5g, ZnSO 4 0.5g, MnSO 4 0.5g, CoCl 2 ·6H 2 O 0.5 g. The composite culture liquid of described Aspergillus niger-Trichoderma viride-Neurospora crassa consists of: every 1L of distilled water contains Aspergillus niger suspension 1mL, Trichoderma viride suspension 1mL, Neurospora crassa suspension 1mL, glucose 10g, soluble starch 15g, peptone 0.75g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 2.5g, MgSO 40.75g. The number of spores in the compou...
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