Method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings

A calla lily group, a technology for breaking dormancy, applied in the field of plant tissue culture, can solve the problems of little or no differentiation, slow growth of seedlings, etc., and achieve the effect of prolonging normal growth and ensuring preservation

Inactive Publication Date: 2020-04-10
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is: in the in vitro preservation of calla lily germplasm resources and the rapid propagation process of seedling tissue culture, the seedlings are prone to degeneration phenomena such as slow growth, little or no differentiation, and the white flower species Z.odorata in autumn and winter The problem with seasonal hibernation

Method used

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  • Method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings
  • Method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings
  • Method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings

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Embodiment 1

[0022] A method for renewing and rejuvenating calla lily tissue culture seedlings and breaking dormancy, specifically comprising the following steps:

[0023] (1) Preparation of TDZ mother liquor and 6-BA mother liquor: Weigh 10mg TDZ and dissolve it in 100mL distilled water (final concentration is 0.1g / mL), filter in a sterile operating bench with a 0.2μm filter membrane, after the sterilization Transfer to a sterile 10mL centrifuge tube and store in a 4°C refrigerator for later use; weigh 10mg of 6-BA, dissolve it in 100mL of distilled water after alcohol-assisted dissolution (final concentration is 0.1g / mL), transfer to a brown bottle, and place Store in a 4°C refrigerator for later use.

[0024] (2) Culture medium preparation: Prepare the mother liquor according to the MS basic medium formula, add TDZ mother liquor to the middle of the mother liquor to make the TDZ concentration 0.01mg / L, add 6-BA mother liquor to the middle of the mother liquor to make the 6-BA concentrat...

Embodiment 2

[0034] A method for renewing and rejuvenating calla lily tissue culture seedlings and breaking dormancy, specifically comprising the following steps:

[0035](1) Preparation of TDZ mother liquor and 6-BA mother liquor: Weigh 10mg TDZ and dissolve it in 100mL distilled water (final concentration is 0.1g / mL), filter in a sterile operating bench with a 0.2μm filter membrane, after the sterilization Transfer to a sterile 10mL centrifuge tube and store in a 4°C refrigerator for later use; weigh 10mg of 6-BA, dissolve it in 100mL of distilled water after alcohol-assisted dissolution (final concentration is 0.1g / mL), transfer to a brown bottle, and place Store in a 4°C refrigerator for later use.

[0036] (2) Culture medium preparation: Prepare the mother liquor according to the MS basic medium formula, add TDZ mother liquor to the middle of the mother liquor, so that the concentration of TDZ is 0.01mg / l, add 6-BA mother liquor to the middle of the mother liquor, so that the concentr...

Embodiment 3

[0041] A method for renewing and rejuvenating calla lily tissue culture seedlings and breaking dormancy, specifically comprising the following steps:

[0042] (1) Preparation of TDZ mother liquor and 6-BA mother liquor: Weigh 10mg TDZ and dissolve it in 100mL distilled water (final concentration is 0.1g / mL), filter it in a sterile operating bench with a 0.2um filter membrane, after the sterilization Transfer to a sterile 10mL centrifuge tube and store in a 4°C refrigerator for later use; weigh 10mg of 6-BA, dissolve it in 100mL of distilled water after alcohol-assisted dissolution (final concentration is 0.1g / mL), transfer to a brown bottle, and place Store in a 4°C refrigerator for later use.

[0043] (2) Culture medium preparation: Prepare the mother liquor according to the MS basic medium formula, add TDZ mother liquor to the middle of the mother liquor, make the concentration of TDZ be 0.01mg / L, add 6-BA mother liquor to the middle of the mother liquor, 6-BA makes the conc...

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Abstract

The invention discloses a method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings and belongs to the technical field of plant tissue culture. The method comprises the following step of inoculating degenerated or dormant common callalily tissue culture seedlings to a special culture medium, wherein the culture medium is prepared by preparing a motherliquor according to an MS basic culture medium formula, and adding 0.008-0.015mg / L of TDZ, 0.05-0.20mg / L of 6-Ba, 28-35g / L of sucrose and 6.20-6.50g / L of agar powder in the mother liquor, and a pH value of the culture medium is adjusted to be between 6.0 and 6.1. By adopting the method disclosed by the invention, the ability of restoring differentiation and robust growth of the degraded common callalily tissue culture seedlings can be promoted, dormancy of the common callalily is broken, the preservation period of the common callalily is prolonged, and the effective technical support is provided for preservation and seedling production of common callalily resources.

Description

technical field [0001] The invention relates to a method for rejuvenating and breaking dormancy of calla lily tissue culture seedlings, which belongs to the technical field of plant tissue culture. Background technique [0002] Calla lily (Zantedeschia spp) is a perennial herbaceous bulbous flower of the genus Zantedeschia in the family Araceae. It has a unique flower shape, bright colors, and both flowers and leaves. It can not only be used as cut flowers and potted flowers, but also can be planted in gardens, Parks, etc., have extremely high ornamental and commercial value, are favored by many consumers, and enjoy the reputation of "flower star" in the 21st century. [0003] Calla lilies are divided into two groups: white-flowered calla lilies (Zantedeschia) and colorful flowered calla lilies (Aestivae). The white-flowered calla lilies include Z.aethiopica and Z.odorata, and the colorful flowered calla lilies include Z.aethiopica, Z.pentlandi, There are six species of Z.e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 吴红芝郭彦兵吴景芝贺水莲金寿林周雯
Owner YUNNAN AGRICULTURAL UNIVERSITY
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