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Artificial propagation method and application of psylla chinensis parasitic wasps

A technology of Psyllium psyllid and parasitoid, which is applied in the field of artificial propagation of Psyllium psyllid parasitoid, can solve problems such as being in the blank, and achieve the effects of small space occupation, avoiding pesticide residues, and easy operation

Inactive Publication Date: 2020-04-07
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the indoor artificial breeding method for the parasitic wasps of pear psyllid is still blank

Method used

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  • Artificial propagation method and application of psylla chinensis parasitic wasps
  • Artificial propagation method and application of psylla chinensis parasitic wasps

Examples

Experimental program
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Effect test

Embodiment 2

[0037] 3.1 The preference of the parasitoid wasps to different ages of Psyllid

[0038] Prepare a 9cm petri dish, prepare 2% water agar, pour it into the petri dish, the thickness is about 3mm, take the third and fourth leaves of pear seedlings, insert the petiole into the agar for moisturizing, and connect the leaves from one instar to Ten fifth instar psyllid nymphs each introduced a pair of parasitoids within 24 hours of emergence, sealed with plastic wrap, and pricked 50 small holes in the plastic wrap with a No. 1 insect needle for ventilation. Put it into an incubator, remove the parasitic wasps after 24 hours, continue to cultivate, and observe the number of parasitic psyllids of each age, a total of 8 repetitions. The result is as figure 1 ,Depend on figure 1 It can be seen that the parasitoid wasps prefer to parasitize the fourth and fifth instar psyllid nymphs but not the first to third instar psyllid nymphs. Therefore, selecting four to five instar psyllid nymphs...

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Abstract

The invention provides an artificial propagation method and application of psylla chinensis parasitic wasps, and the method comprises the following steps: collecting psylla chinensis nymphs which havebeen spawned and parasitized by psylla chinensis parasitic wasps in a pear orchard together with pear leaves, artificially culturing the psylla chinensis nymphs until the psylla chinensis nymphs emergence; mixing newly emerged male and female adult psylla chinensis parasitic wasps, inoculating the mixture into an insect breeding cage containing pear seedlings with fourth-instar psylla chinensis nymphs and fifth-instar psylla chinensis nymphs, feeding with honey water, mating to lay eggs, taking out the pear seedlings after 3 days, transferring to another insect breeding room, continuously breeding, and observing the development state of psylla chinensis nymphs day by day; picking a single psylla chinensis nymph which has been spawned and parasitized by parasitic wasps into a centrifuge tube, artificially culturing, after eclosion of the psylla chinensis nymph, mixing newly eclosion psylla chinensis male and female adults, inoculating into a new insect breeding cage with pear seedlingsof fourth-instar and fifth-instar psylla chinensis nymph, feeding with honey water, mating and spawning. Reciprocating as such, the continuous and stable number of psylla chinensis parasitic wasps are obtained.

Description

technical field [0001] The invention belongs to the field of artificial breeding of natural enemy insects, and in particular relates to a method for artificially multiplying parasitoids of the pear psyllid and its application. Background technique [0002] Cacopsylla chinensis (Cacopsylla chinensis) belongs to Hemiptera, Psyllidae, commonly known as Chinese pear psyllid, is a dangerous piercing-sucking pest that seriously damages pear trees. Although its size is small and its feeding habits are relatively single, it can feed on a variety of pear tree varieties, and both adults and nymphs can cause serious damage. The fungus, mainly caused by the direct sucking of the adults and the weakening of the tree vigor and the indirect secretion of honeydew by the nymphs, caused damage to the trees, resulting in dry leaves and a serious decline in fruit quality and yield. Direct damage and indirect damage affect the growth of pear trees and fruit quality together. For a long time, p...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G17/00
CPCA01G17/005A01K67/033
Inventor 石旺鹏秦紫芳张柳俸明月曹凯丽
Owner CHINA AGRI UNIV
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