Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rapid high-selectivity hypobromous acid fluorescence probe, and preparation method and application thereof

A fluorescent probe and sulfonic acid group technology, applied in the field of fluorescent probes, can solve the problems of lack of fluorescent probes, low concentration, short oxidation reaction time, etc., and achieve the effects of strong anti-interference ability, good stability and sensitive response.

Active Publication Date: 2020-03-13
BIOLOGY INST OF SHANDONG ACAD OF SCI
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, fluorescent probes for the detection of hypobromous acid in living organisms are relatively scarce, and due to the short oxidation reaction time and low concentration of hypobromous acid under physiological conditions, it is necessary to explore a new type of high-efficiency fluorescent probe for the rapid detection of hypobromous acid still a hot issue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid high-selectivity hypobromous acid fluorescence probe, and preparation method and application thereof
  • Rapid high-selectivity hypobromous acid fluorescence probe, and preparation method and application thereof
  • Rapid high-selectivity hypobromous acid fluorescence probe, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the synthesis of formula (II) compound

[0048] The synthetic design route is as follows:

[0049]

Embodiment approach 1

[0050] Embodiment 1: Dissolve 431.5mg (1.5mmol) of N-butyl-4-chloro-1,8-naphthalimide in 8mL of ethylene glycol methyl ether, and then add 301.5mg (1.5mmol) of 1-(2 -aminoethyl)piperidine dihydrochloride, then N,N-diisopropylethylamine was added to reflux for 10h, and then rotary evaporation was carried out by a rotary evaporator to obtain a crude product. If want to obtain purer product, can carry out chromatographic column separation to obtain pure product, obtain pure product 284mg, productive rate is 50%.

Embodiment approach 2

[0051] Embodiment 2: Dissolve 431.5mg (1.5mmol) of N-butyl-4-chloro-1,8-naphthalimide in 8mL of ethylene glycol methyl ether, and then add 603mg (3mmol) of 1-(2-ammonia Ethyl) piperidine dihydrochloride, then reflux for 10h, and then use a rotary evaporator to carry out rotary evaporation to obtain a crude product. If want to obtain purer product, can carry out chromatographic column separation to obtain pure product, obtain pure product 370mg, productive rate is 65%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rapid high-selectivity hypobromous acid fluorescence probe, and a preparation method and an application thereof. The fluorescence probe is a 1-(2-aminoethyl)piperidine compound, and can be used for measuring, detecting or screening hypobromite and carrying out living cell fluorescence imaging as a hypobromite fluorescence probe. The probe can realize at least one of high-selectivity identification of hypobromite, rapid response to hypobromite, ultra-sensitive analysis of hypobromite and detection of hypobromite under physiological level conditions, and also has the advantages of strong anti-interference capability, simplicity in synthesis, and stable properties.

Description

technical field [0001] The invention belongs to the field of fluorescent probes, in particular to a fluorescent probe of 1-(2-aminoethyl)piperidine compounds and its application in measuring, detecting or screening hypobromous acid and live cell fluorescence imaging methods; The invention also provides a method for preparing the fluorescent probe. Background technique [0002] Hypobromous acid (HBrO) is an integral part of biological host defense systems and has chemical and physical properties very similar to hypochlorous acid. Endogenous hypobromous acid is produced by H 2 o 2 with Br － It is formed by the reaction catalyzed by eosinophil peroxidase (EPO). In the body, hypobromous acid is a strong oxidizing agent with effective antibacterial activity, however, excessively produced hypobromous acid can damage organisms and cause tissue damage, and can lead to many diseases such as arthritis, cardiovascular disease, cancer and Asthma etc. The correlation of EPO in the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D401/12C09K11/06G01N21/64
CPCC07D401/12C09K11/06C09K2211/1029G01N21/6428G01N21/6486
Inventor 盛文龙刘可春李晓彬王荣春夏青侯海荣
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com