Primer, system and kit for MET gene amplification detection

Abstract

The invention discloses a primer group for MET gene amplification detection, a kit and a related medication guidance system, and is characterized by constructing calibration plasmids and standard plasmids for correcting errors of primers and an amplification system, and the accuracy of the kit is improved. A 2-Ct method is adopted, is suitable for gene copy number detection of diploid animals, andis also suitable for determining the copy number of a certain gene of polyploid animals, plants, microorganisms and the like, so that the problem that PCR methods are not easy to detect the gene copynumber is solved. The method is simple and easy to operate, a result is easy to interpret, and a very beneficial thought is provided for other application of gene copy number detection (such as geneamplification and chromosome structural variation).

Description

technical field [0001] The invention relates to the field of biological technology, and relates to a primer set, system and kit for MET gene amplification detection. Background technique [0002] The morbidity and mortality of lung cancer rank first among malignant tumors in the world and in my country, and it is the main cause of cancer-related death. About 85% to 90% of lung cancers are non-small cell lung cancer (NSCLC). For patients with non-small cell lung cancer (NSCLC) with EGFR mutations, epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) Gefitinib and erlotinib are effective drugs for this type of lung cancer. [0003] Despite the enormous benefit of EGFR TKIs in EGFR-mutated non-small cell lung cancer (NSCLC), patients eventually developed resistance to both gefitinib and erlotinib (known as "acquired resistance"), studies have shown. Medicinal properties"). [0004] Two mechanisms of acquired resistance have been identified. The first me...

AI Technical Summary

Problems solved by technology

Moreover, the quantitative method in the present invention is not only applicable to the gene copy number detection of diploid animals, but also applicable to polyploid animals, plants, microorganisms, etc. to determine the copy number of a certain gene, thus solving the problem that the PCR method is not easy to detect. The problem of gene copy number, and this method is simple, easy to operate, and easy to interpret the results. Compared with other methods, it has very obvious advantages, and provides a very beneficial application for other detection of gene copy number (such as gene amplification, chromosome structure variation) train of thought

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