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Preparation of composite fluorescent nanoprobe and method for detecting hydrogen peroxide by using composite fluorescent nanoprobe

A nanoprobe and composite fluorescence technology, applied in the field of nanoprobes, can solve the problems of long reaction detection time, low selection specificity, detection environment interference, etc., achieve good detection selectivity, efficient detection goals, and reduce preparation costs Effect

Active Publication Date: 2020-02-25
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the GB / T23499-2009 national standard, there are iodometric method and titanium salt colorimetric method about the methods for measuring the residual amount of hydrogen peroxide. Defects such as long detection time, inconvenient use for trace detection, slow response, and susceptibility to interference from the detection environment

Method used

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  • Preparation of composite fluorescent nanoprobe and method for detecting hydrogen peroxide by using composite fluorescent nanoprobe
  • Preparation of composite fluorescent nanoprobe and method for detecting hydrogen peroxide by using composite fluorescent nanoprobe
  • Preparation of composite fluorescent nanoprobe and method for detecting hydrogen peroxide by using composite fluorescent nanoprobe

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Effect test

Embodiment 1

[0043] Embodiment 1: the synthesis of CdSe@ZnS quantum dots

[0044] CdCl 2 2H 2 Dissolve O (38.4 mg) and NAC (27.5 mg) in 80 mL of ultrapure water, mix and stir evenly, adjust the pH of the solution to 9 with NaOH, add 170 μL of NaHSe solution, stir at room temperature for 2 h, and transfer the reaction solution to 100 °C Heated for 6 h, then added 3 mL of Zn(OAc) 2 2H 2 O and 3 mL of Na 2 S·9H 2 O solution to obtain CdSe@ZnS quantum dots.

[0045] Wherein, the concentration of NaOH is 1mol / L, NaHSe solution is made of Se powder (39.5mg) and NaBH 4 (40mg) was reacted in 2mL ultrapure water, Zn(OAc) 2 2H 2 O and Na 2 The concentrations of S were 11.5 mg / mL and 12 mg / mL, respectively.

[0046] The perspective electron microscope picture is as follows figure 1 As shown, the as-prepared CdSe@ZnS quantum dots show good dispersion and are spherical in shape, and the corresponding Gaussian fitting results of the histograms obtained from the TEM images of CdSe@ZnS quantum do...

Embodiment 2

[0047] Embodiment 2: Synthetic AgNCs

[0048] Weigh 52.6 mg of DL-lipoic acid and dissolve it in 20 mL of ultrapure water, add 400 μL of NaOH solution (1mol / L), stir until completely dissolved, then add 2 mL of NaBH 4 (0.24mg / mL) solution and 0.5mL of AgNO 3 (25mM) solution to prepare nano-silver clusters.

[0049] The perspective electron microscope picture is as follows figure 2 As shown, the synthesized nano-silver clusters are spherical, and the dispersion is good, and its particle size is 1.7 ± 0.5nm.

Embodiment 3

[0050] Example 3: Construction of CdSe@ZnS / AgNCs composite fluorescent nanoprobe

[0051] 20 μL of CdSe@ZnS quantum dot solution (3.2 mg / mL) and 20 μL of AgNCs solution (1.5 mg / mL) were diluted to 2 mL with Tris-HCl (pH=7.4) solution and incubated together for 10 min to prepare CdSe@ZnS ZnS / AgNCs composite fluorescent nanoprobes.

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Abstract

The invention discloses a preparation method of a CdSe@ZnS / AgNCs composite fluorescent nanoprobe. CdSe@ZnS quantum dots are mixed with nano-silver clusters under a certain pH condition to prepare theCdSe@ZnS / AgNCs composite fluorescent nanoprobe with the CdSe@ZnS quantum dot concentration of 0.032 mg / mL and the nano-silver cluster concentration of 0.015 mg / mL. The CdSe@ZnS / AgNCs composite fluorescent nanoprobe constructed by directly mixing the CdSe@ZnS quantum dots and nano-silver clusters has the advantage of simple synthesis method. The constructed CdSe@ZnS / AgNCs composite fluorescent nanoprobe realizes oxidative degradation of the silver clusters through hydrogen peroxide to release silver ions, and the silver ions have a quenching effect on fluorescence of the CdSe-ZnS quantum dots,so the efficient detection target of hydrogen peroxide is achieved, thereby the technical problems of long detection reaction time, slow response and insufficient low detection limit of traditional technologies are solved.

Description

technical field [0001] The invention relates to the technical field of nanoprobes, more specifically, the invention relates to the preparation of a composite fluorescent nanoprobe and its detection method for hydrogen peroxide. Background technique [0002] In recent decades, food safety issues have attracted people's attention more and more, and food safety issues have also been put on the agenda. The rich nutrients in food provide a good living environment for the growth of microorganisms. Hydrogen peroxide has an efficient fresh-keeping function on food, so people have developed food additive hydrogen peroxide in order to pursue the freshness of food. Hydrogen peroxide can inhibit the growth of microorganisms in food, so as to achieve the purpose of food preservation for a long time, but the residual hydrogen peroxide in food will seriously endanger human health. As one of the important members of active oxygen, hydrogen peroxide can cause DNA damage and aging of the bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/88B82Y30/00G01N21/64
CPCC09K11/883C09K11/02B82Y30/00G01N21/643G01N2021/6432
Inventor 周志强杨立云黄凌刘义
Owner GUANGXI TEACHERS EDUCATION UNIV
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