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Method used for detecting content of coumarin components in angelica dahurica medicinal material on basis of UPLC and quantitative analysis of multiple components by a single marker

A technology for coumarins and content, which is used in measurement devices, material separation, and analysis of materials, etc., can solve the problems of low efficiency and long detection time, and achieve the effects of high accuracy, short detection time, and low detection cost.

Inactive Publication Date: 2020-02-21
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been many reports on the determination of coumarin components in Baizhi medicinal materials, but they are all based on HPLC and external standard method determination, such as Wang Mengyue. The ancient and modern medicinal situation of Baizhi and the study of coumarin components[D]. Chengdu: Chengdu University of Traditional Chinese Medicine, 2003.33‐39. The HPLC external standard method was used to determine the main coumarin components imperatorin, isoimperatorin, and hydrated oxidized imperatorin in Baizhi, but the detection time was long and the efficiency was low. not tall

Method used

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  • Method used for detecting content of coumarin components in angelica dahurica medicinal material on basis of UPLC and quantitative analysis of multiple components by a single marker
  • Method used for detecting content of coumarin components in angelica dahurica medicinal material on basis of UPLC and quantitative analysis of multiple components by a single marker
  • Method used for detecting content of coumarin components in angelica dahurica medicinal material on basis of UPLC and quantitative analysis of multiple components by a single marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 Detection method of the present invention

[0025] 1. Detection method

[0026] (1) Preparation of mixed reference substance

[0027] Take the reference substances of imperatorin, oxidized precurin, isoimperatorin and hydrated oxidized precurin respectively, weigh them accurately, put them in a 10mL measuring bottle, add methanol to dissolve and dilute to make each 1mL containing 11.9, 14.7 , 10.4, 5.5 μg of mixed reference solution. Accurately draw 0.1, 0.2, 0.4, 0.8, 1, 2, and 4 mL of the mixed reference substance solution respectively, place them in a 10 mL measuring bottle, add methanol to dilute to the mark, and shake well to obtain a series of mixed reference substance solutions for future use.

[0028] (2) preparation of need testing solution

[0029] Take 0.5g of Angelica dahurica powder (passed through a No. 3 sieve), weigh it accurately, put it in a 50mL measuring bottle, add 45mL of methanol, ultrasonically treat it for 1 h, take it out, let i...

Embodiment 2

[0034] Embodiment 2 Detection method of the present invention (one test and multiple evaluations)

[0035] 1. Detection method

[0036] (1) Preparation of mixed reference substance

[0037] Take the imperatorin reference substance, accurately weigh it, place it in a 10mL measuring bottle, add methanol to dissolve and dilute to make a reference substance solution containing 11.9μg per 1mL. Accurately draw reference substance solution 0.1, 0.2, 0.4, 0.8, 1, 2, 4mL respectively into a 10mL measuring bottle, add methanol to dilute to the mark, shake well to obtain a mixed reference substance solution, and set aside.

[0038] (2) preparation of need testing solution

[0039] Take 0.5g of Angelica dahurica powder (passed through a No. 3 sieve), weigh it accurately, put it in a 50mL measuring bottle, add 45mL of methanol, ultrasonically treat it for 1 h, take it out, let it cool, add methanol to the scale, shake well, filter, and take the subsequent filtrate , that is. Filter wit...

Embodiment 3

[0048] Embodiment 3 detection method of the present invention

[0049] Instruments and reagents

[0050] 1 instrument

[0051]Agilengt 1290Infinity LC Ultra High Performance Liquid Chromatograph (Agilent Corporation, USA), SQP 1 / 10,000 Analytical Balance (Germany Sartorius Scientific Instrument Co., Ltd.), BT125D 1 / 100,000 Analytical Balance (Germany Sartorius Scientific Instrument Co., Ltd. ), KQ‐300 ultrasonic cleaning machine (Kunshan Ultrasonic Equipment Co., Ltd.).

[0052] 2 reagents

[0053] Imeratorin (batch number 20170528, mass fraction is 99.65%), oxidized imperatorin (batch number 20170216, mass fraction is 99.89%), isoimperatorin (batch number 20170620, mass fraction is 99.07%), hydrated and oxidized imperatorin Sulfate (batch number 20170426, mass fraction 99.97%) was obtained from Chengdu Ansaisi Biotechnology Co., Ltd.; acetonitrile (U.S. Fisher Company, chromatographically pure); methanol (analytical pure); Watsons distilled water.

[0054] The medicinal m...

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Abstract

The invention provides a method used for detecting the content of coumarin components in an angelica dahurica medicinal material on the basis of UPLC and the quantitative analysis of multiple components by a single marker. According to the method, four components in the coumarin components in the angelica dahurica medicinal material can be detected at the same time on the basis of the quantitativeanalysis of multiple components by the single marker. Compared with a common HPLC detection method, the method has the advantages of better stability, higher accuracy, shorter detection time, higherefficiency and lower detection cost. According to the method adopted, the content of isoimperatorin, oxypeucedanin and oxypeucedanin hydrate is calculated through a relative correction factor f and chromatographic peak positioning under the condition of lack of isoimperatorin, oxypeucedanin and oxypeucedanin hydrate reference substances; and therefore, the measurement of the content of the coumarin components of the isoimperatorin, oxypeucedanin and oxypeucedanin hydrate in the angelica dahurica can be realized, and a new method is provided for the quality control of the angelica dahurica.

Description

technical field [0001] The invention specifically relates to the detection of the content of coumarin components in the medicinal material of Angelica dahurica based on UPLC and the one-test-multiple-evaluation method. Background technique [0002] Baizhi is Angelica dahurica (Fisch.ex Hoffm.) Benth.etHook.f. or Hangbaizhi Angelica dahurica (Fisch.ex Hoffm.) Benth.etHook.f.var.formosana (Boiss.) Shan et Yuan The dry root of the root has a long history of use. It is clinically used for cold headache, brow bone pain, nasal congestion, runny nose, rhinorrhea, nasal sinusitis, toothache, leukorrhea, sore swelling and pain. Studies have shown that the main active ingredients of Angelica dahurica are coumarins, mainly including imperatorin, isoimperatorin, oxidized precurin, hydrated oxidized precurin, white angelica, white angelica brain, bergamot lactone, etc. [0003] At present, the research on the coumarin compounds of Angelica dahurica is also relatively mature, from the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 马逾英马俊曹赟蒋桂华陈玲连艳刘晓芬
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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