Marine bacteria capable of antagonizing two important pathogenic vibrios of marine culture and application of marine bacteria
A technology of mariculture and marine bacteria, which is applied in the direction of antibacterial drugs, bacteria, and medical raw materials derived from bacteria, etc., can solve problems such as threats to human health and safety, fish are prone to drug resistance, and ecological balance is destroyed, and the cost is achieved. Low, high antagonistic activity, stable yield effect
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Embodiment 1
[0013] Example 1 Isolation, Identification and Preservation of Pseudoalteromonas
[0014] The screening method of the above-mentioned antagonistic bacteria is as follows: firstly, the pathogenic bacteria Vibrio anguillarum (Vibrio anguillarum) and alginolyticus (vibrio parahemolyticus) were used as indicator bacteria respectively, and more than 300 strains of bacteria isolated from Antarctic ice seawater and turbot breeding pond water were used, The antagonistic bacteria were screened by spot method and filter paper method. The extracellular products of antagonistic bacteria obtained through screening will be used for the prevention of hemorrhage and ulcer epidemic diseases in turbot fins. The specific technical scheme is as follows:
[0015] Screening of antagonistic bacteria: Inoculate the indicator bacteria Vibrio anguillarum and vibrioparahemolyticus in LB liquid medium containing 3% NaCl respectively, culture with shaking at 28°C for 12-16 hours, take 40μl-50μl Spread t...
Embodiment 2
[0024] Example 2 Application of antagonistic bacteria Pseudoalteromonas agarivorans
[0025] 100 healthy and uniform turbots (average body length 10±1cm, average body weight 12±2g) were raised in a 30L seawater storage tank. During the experiment, the water was continuously aerated at a temperature of 20°C and the water was changed every day. Do not cast bait. Vibrio anguillarum, Vibrio parahemolyticus and Pseudoalteromonas agarivorans isolated by screening were inoculated in LB medium containing 3% NaCl, and cultured at 28°C for 24 hours. Vibrio anguillarum and Vibrio alginolyticus were centrifuged and resuspended in sterile sea water respectively to obtain the bacterial suspension. Pseudoalteromonas was centrifuged to discard the precipitate, and the supernatant was filtered through a 0.22 μm microporous membrane. Carry out the following three groups of experimental treatments, 30 tails in each group; (1) the control group, without adding pathogenic bacteria and antagonisti...
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