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QTL loci and analysis methods controlling traits related to wheat epidermis and stomata

An analysis method and epidermal hair technology, applied in the field of molecular biology, can solve the problems of inaccurate measurement data, large differences in the distribution of epidermal hair and stomata, no analysis of different environments, and different parts, and achieve the effect of accurate measurement methods.

Active Publication Date: 2022-05-17
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing technology is mainly to locate the chromosomes controlling the density of wheat epidermis in a single environment, without analyzing different environments and different parts, and the research on stomata-related traits is mostly limited to the middle part of the leaf, but the epidermal hairs and stomata are located in the leaves. The distribution on the leaves is quite different, and only studying a certain part does not fully represent the characteristics of the whole leaf.
In addition, due to statistical flaws in the measurement of epidermis and stomatal properties, the measurement data is not accurate, which affects the subsequent statistical analysis

Method used

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  • QTL loci and analysis methods controlling traits related to wheat epidermis and stomata
  • QTL loci and analysis methods controlling traits related to wheat epidermis and stomata
  • QTL loci and analysis methods controlling traits related to wheat epidermis and stomata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Effect of Drought Stress on Wheat Leaf Epidermis-related Traits

[0045] (1) Experimental materials:

[0046] ①Hanxuan 10; ②Lumai 14; ③With Hanxuan 10 as the female parent and Lumai 14 as the male parent, a wheat DH population with 150 lines was established through anther culture.

[0047] (2) Material planting:

[0048]On September 25, 2011, it was sown on-demand in the experimental field of the Agricultural Station of the Agricultural College of Shanxi Agricultural University, with a row length of 2m, 40 grains per row, and a double-row area. The soil is sandy loam, and the 0-20cm soil layer of the test site contains 15.6g / kg of organic matter, 2.8g / kg of total nitrogen, 10.4mg / kg of available phosphorus, and 126.7mg / kg of available potassium. Water management was divided into two treatments: drought stress (Drought-Stress, DS) and normal irrigation (Well-Watered, WW). Each treatment was repeated 3 times, and the random block design was used. Before sowin...

Embodiment 2

[0061] Example 2 Effects of Drought Stress on Wheat Leaf Stomata-Related Characters

[0062] (1) Experimental materials and (2) material planting are the same as in Example 1.

[0063] (3) Measurement and calculation of stomatal properties:

[0064] ①Sampling, ②Sample preparation operation steps are the same as in Example 1.

[0065] ③Measurement and calculation: select 3 visual fields at the edge, middle (between leaf edge and main vein) and main vein edge of each leaf tip, leaf middle and leaf base, a total of 27 visual fields at 100 times Observe and take pictures, and use the SmileView measurement software to measure the number of stomata (then convert to stomatal density, number / mm 2 ), pore length (μm) and pore width (μm).

[0066] ④ Data processing: Data correlation analysis was processed with SPSS17.0 software.

[0067] (4) Experimental results: the experimental results are shown in Table 3-5, Figure 3-5 shown.

[0068] Table 3 Comparison of stomatal density in ...

Embodiment 3

[0078] Example 3 QTL analysis of traits related to wheat leaf epidermis

[0079] (1) Genetic linkage map:

[0080] The linkage map of the DH population was constructed by the Key Laboratory of Crop Germplasm Resources and Biotechnology of the Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences. It has a total length of 3904cM and contains 395 markers. There are 19 markers, but the number of marker sites on different chromosomes is quite different. There are 161 markers in genome A, 177 markers in genome B, and 57 markers in genome D, accounting for 40.8%, 44.8% and 14.4% of the total markers respectively .

[0081] The construction method of the linkage map of the DH population:

[0082] ① Select DNA markers suitable for mapping; ② According to the DNA polymorphism among genetic materials, select parental combinations for establishing mapping populations; ③ Establish segregated populations with a large number of DNA markers in a segre...

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Abstract

The invention discloses a group of QTL sites and an analysis method for controlling traits related to wheat epidermis and stomata, belonging to the field of molecular biology. The QTL loci include Qtd‑2A‑1, Qtd‑2B‑1, Qtd‑7D‑1, Qt l‑7D‑1, Qt l‑7D‑2, Qsl‑6A‑1, Qsl‑5A‑2 and Qsl -4A. The QTL analysis methods are: (1) construction of wheat DH population; (2) collection of phenotypic data of wheat epidermis and stomata related traits under normal irrigation and drought stress; (3) using genetic linkage map, combined with Phenotypic data were subjected to QTL analysis. The beneficial effects of the present invention are: to study the influence of drought stress conditions on the traits related to epidermal hairs and stomata, and to carry out QTL analysis on the traits related to epidermal hairs and stomata in different parts of the leaves under two kinds of water conditions, and to explore the stable expression in different environments. QTL loci.

Description

technical field [0001] The invention relates to the technical field of molecular biology, and more specifically relates to a group of QTL sites and an analysis method for controlling traits related to wheat epidermis and stomata. Background technique [0002] Wheat is one of the most important food crops in the world. Drought is the most important abiotic stress factor affecting wheat yield. Therefore, research on drought-resistant genetics and breeding of wheat is particularly important. It is of great significance to study the genetic basis of drought-resistance-related traits in wheat, further analyze the molecular mechanism of crop drought-resistance, and search for molecular markers closely linked to drought-resistance. [0003] Drought stress is currently the most important abiotic limiting factor limiting the increase in wheat yield. Leaf trichomes and stomata are ecological adaptive choices in the process of crop evolution. Under drought stress, crops will adapt to t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 范华孙黛珍王曙光史雨刚
Owner SHANXI AGRI UNIV
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