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Oocystis algae culture process

A cultivation process and technology of oocysts, which are applied in the field of aquaculture water quality control and water environment management, can solve the problems of inapplicable expansion stage, insufficient stability of oocyst algal phase, and easy pollution, so as to shorten the time of cultivation and expansion , not easy to aging and anti-algae, strong adaptability to algae

Pending Publication Date: 2020-02-07
NINGBO FUTIAN BIOTECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] However, the traditional oocyst cultivation method adopts open cement pond cultivation, which has a large scale of cultivation, but its disadvantages are: (1) It is easy to pollute, occupies a large area, is greatly affected by the weather, and is not easy to control, and the algae cells grow slowly and multiply The time is long, the one-time investment is large and the output is low, and it is not suitable for the expansion stage; (2) the algal phase of the oocysts cultivated by the traditional method is not stable enough, and it is easy to age and lose algae. In the middle and late stages, algae collapse is easy to occur, and the dead algae will produce a large amount of algae toxins, which will lead to unstable water quality in the pond, which will lead to the death of seafood such as prawns; It is inconvenient to use for long-term storage and transportation, which makes it difficult for oocysts to be applied on a large scale in actual production, and needs to be improved

Method used

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  • Oocystis algae culture process

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Experimental program
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Effect test

Embodiment 1

[0031] In the present embodiment, oocysts adopt ultraviolet radiation mutagenesis method, specifically:

[0032] a. Add sodium acetate to Zhanshui 107-13 medium until the concentration reaches 10g / L, inoculate oocysts, and dilute to 10 3 -10 4 Cells / mL, in the ultra-clean bench, place the algae liquid in a large petri dish with a diameter of 20cm, the thickness of the algae liquid does not exceed 0.5cm, and treat it with 8W UVB at a height of 15-20cm for 30min.

[0033] The treated algae liquid was inoculated into the solid Zhanshui 107-13 plate containing 10g / L NaAc, and the normally growing oocyst algae strains were screened out.

[0034] Then the above-mentioned oocyst algae strains are activated, and the above-mentioned obtained algae strains are inoculated into the sterilization reactor for ventilation and light cultivation, the light intensity is 500-700Lx, the ventilation volume is 0.2vvm, and the cultivation time is 4d, until the concentration of the oocyst algae seed...

Embodiment 2

[0038] In the present embodiment, oocysts adopt the ultraviolet radiation mutagenesis method described in Example 1, and then 310*10 4 Oocyst algae seed solution at a concentration of 100L / mL was inoculated into a 100L fermenter for heterotrophic culture, and the initial inoculated seed amount was 10%, accounting for the mass ratio of the medium.

[0039] The medium in the fermenter includes the following concentration components: 3g / L sodium acetate, 0.5g / L potassium nitrate, 0.08g / L potassium dihydrogen phosphate, 0.08g / L ferric chloride, VB1: 0.003g / L, VB12: 0.0002g / L.

[0040] Fed-batch fermentation, feed nutrient salt on the second day after inoculation, including the following concentration components: sodium acetate 300g / L, potassium nitrate 50g / L, potassium dihydrogen phosphate 8g / L, ferric chloride 8g / L, VB1 0.3g / L, VB12 0.02g / L, the amount of the whole feeding nutrient salt is about 30% of the fermented algae liquid.

[0041] Fermentation process parameters: pH in...

Embodiment 3

[0045]In the present embodiment, oocysts adopt the ultraviolet radiation mutagenesis method described in Example 1, and then 350*10 4 Oocyst algae seed solution at a concentration of / mL was inoculated into a 100L fermenter for heterotrophic culture, and the initial inoculated seed amount was 8%, accounting for the mass ratio of the medium.

[0046] The medium in the fermenter includes the following concentration components: 2.2g / L sodium acetate, 0.4g / L potassium nitrate, 0.05g / L potassium dihydrogen phosphate, 0.06g / L ferric chloride, VB1: 0.002g / L, VB12 : 0.00015g / L.

[0047] Fed-batch fermentation, feeding nutrient salt on the second day after inoculation, including the following concentration components: sodium acetate 220g / L, potassium nitrate 40g / L, potassium dihydrogen phosphate 5g / L, ferric chloride 6g / L, VB1-0.15 g / L, VB12-0.015g / L, the amount of the whole feeding nutrient salt is about 35% of the fermented algae liquid.

[0048] Fermentation process parameters: pH...

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Abstract

The invention provides an oocystis algae culture process. According to the culture process, a high-concentration sodium acetate resistant oocystis algae strain is produced by mutagenesis, the cultureprocess is improved in a targeted manner, a fermentation tank culture process is adopted, components of a culture medium are changed, and finally a photoadaptive culture is carried out, so that the culture cycle of oocysis algae seed liquid is greatly shortened, the culture process is suitable for seed expansion, the cultured algae strain is stable in growth state of algae and is not prone to aging and falling, and the oocyst algae seed liquid is suitable for long-time storage and transportation.

Description

technical field [0001] The invention relates to the technical field of aquaculture water quality control and water body environment treatment, in particular to a process for cultivating oocysts. Background technique [0002] Oocysts are mainly distributed in water bodies rich in organic matter such as estuaries and aquaculture ponds. They have the characteristics of long duration and stable population. They can reduce the concentration of harmful factors such as ammonia nitrogen and nitrite in water bodies, and increase dissolved oxygen in water. , so that the water environment is in a benign balance and inhibit the growth of Vibrio. Because of its characteristics, oocysts are widely found in southern prawn culture ponds, which can regulate and improve the culture environment and prevent prawn diseases. [0003] However, the traditional oocyst cultivation method adopts open cement pond cultivation, which has a large scale of cultivation, but its disadvantages are: (1) It is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N13/00C12R1/89
CPCC12N1/12C12N13/00
Inventor 王兆伟
Owner NINGBO FUTIAN BIOTECH
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