Virgin olive oil control fingerprint spectrum and construction method and application thereof
A technology for comparing fingerprints and virgin olive oil, which is applied in the detection field and can solve the problems of large range of adulteration types and adulteration amounts, misjudgment, etc.
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[0091] 1.1 Establishment of the control fingerprint of virgin olive oil
[0092] 1.1.1. Take 10.0g of 17 batches of extra virgin olive oil, weigh them precisely, put them in a 50mL polyethylene tube, add 20mL of methanol precisely, vortex extract for 1.0min, centrifuge at 10000r / min, and take 1.5mL of the supernatant , and filtered through a 0.22 μm organic phase microporous membrane to obtain 17 extra virgin olive oil samples.
[0093] 1.1.2. Perform ultra-high performance liquid chromatography-fluorescence detection on 17 extra virgin olive oil samples, and obtain the chromatographic information of 17 extra virgin olive oil samples. figure 1 Overlapping chromatogram information of 9 extra virgin olive oil samples among 17 extra virgin olive oil samples;
[0094] 1.1.3. Calculate the similarity of the chromatographic information of 17 extra virgin olive oil samples, obtain the comparison fingerprint of virgin olive oil, and set the chromatographic peaks with a peak area grea...
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