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Virgin olive oil control fingerprint spectrum and construction method and application thereof

A technology for comparing fingerprints and virgin olive oil, which is applied in the detection field and can solve the problems of large range of adulteration types and adulteration amounts, misjudgment, etc.

Active Publication Date: 2020-02-04
LONGNAN XIANGYU OIL OLIVES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some studies have shown that the fatty acid composition of some vegetable oils is relatively similar, so there is a certain risk in identifying and identifying these oils with similar fatty acid composition through fatty acid fingerprints, and the types and amounts of adulteration are also relatively large, so only Possibility of Misjudgment Using Fatty Acid Fingerprints to Identify Adulterated Oils

Method used

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  • Virgin olive oil control fingerprint spectrum and construction method and application thereof
  • Virgin olive oil control fingerprint spectrum and construction method and application thereof
  • Virgin olive oil control fingerprint spectrum and construction method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0091] 1.1 Establishment of the control fingerprint of virgin olive oil

[0092] 1.1.1. Take 10.0g of 17 batches of extra virgin olive oil, weigh them precisely, put them in a 50mL polyethylene tube, add 20mL of methanol precisely, vortex extract for 1.0min, centrifuge at 10000r / min, and take 1.5mL of the supernatant , and filtered through a 0.22 μm organic phase microporous membrane to obtain 17 extra virgin olive oil samples.

[0093] 1.1.2. Perform ultra-high performance liquid chromatography-fluorescence detection on 17 extra virgin olive oil samples, and obtain the chromatographic information of 17 extra virgin olive oil samples. figure 1 Overlapping chromatogram information of 9 extra virgin olive oil samples among 17 extra virgin olive oil samples;

[0094] 1.1.3. Calculate the similarity of the chromatographic information of 17 extra virgin olive oil samples, obtain the comparison fingerprint of virgin olive oil, and set the chromatographic peaks with a peak area grea...

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Abstract

The invention provides a virgin olive oil control fingerprint spectrum and a construction method and application thereof, and belongs to the technical field of detection. A series of extra virgin olive oils are analyzed by using ultra-high performance liquid chromatography-fluorescence detection, thereby obtaining chromatographic information of various components in the extra virgin olive oils, breaking through the limitations of constructing a fingerprint spectrum by a traditionally used GC-MS method and based on the fatty acid composition of edible oil, and further solving the problem that for a traditional fingerprint spectrum, only a single component is used for discrimination and causes false positive. At the same time, similarity calculation is performed on the chromatographic information of the series of extra virgin olive oils, and the virgin olive oil control fingerprint spectrum is established, thereby strengthening the polymorphism of the fingerprint spectrum information, reflecting differences from the edible oil from multiple perspectives of the substance composition, distribution, and content of virgin olive oil, being conducive to improving the accuracy of identification, and being capable of conveniently, quickly and accurately identifying the quality of the virgin olive oil.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a comparison fingerprint of virgin olive oil and its construction method and application. Background technique [0002] Olive oil refers to the oil obtained from fresh olive fruit (not including the oil obtained by solvent extraction or heavy esterification), and is not mixed with other types of oil. Virgin olive oil has the highest quality and nutritional value. It is rich in unsaturated fatty acids, squalene, polyphenols, vitamins and other natural active substances. It has anti-oxidation, cholesterol regulation, cancer prevention, beauty and other effects. The price is lower than other foods. Vegetable oil is much higher. Because olive oil is favored by people for its unique physical and chemical properties and excellent nutritional and health benefits, some businessmen are driven by economic interests, and the problem of olive oil adulteration has always been serious. At ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/36G01N30/74G01N30/86G01N21/64
CPCG01N30/02G01N30/34G01N30/36G01N30/74G01N21/64G01N30/8686G01N2030/027G01N33/03G01N2030/8813G01N1/38
Inventor 王波刘玉红李建科金凤周小平杨永青吴龚鹏赵敏王慧珺张欢
Owner LONGNAN XIANGYU OIL OLIVES DEV
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