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Takifugu obscurus SNP molecular markers and application thereof in genetic breeding

A technology of puffer obscurus and molecular markers, which is applied in the direction of recombinant DNA technology, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of few edible parts, short trunk, and no release, etc., and achieve The results are accurate and stable, the data capacity is huge, and the conditions and results are clear and intuitive.

Active Publication Date: 2020-02-04
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of the artificial breeding process of puffer obscurus, the breeding of its fine species has gradually entered the right track, but there are still many problems: although the appropriate breeding density and sufficient bait have been ensured, there are still many individuals that appear similar to Other individuals in the same period have a large difference in growth, and this is mostly reflected in body weight; although some individuals have little difference in weight from other individuals, their trunks are short, which means that there are not many edible parts; under natural conditions, puffer obscurus The natural water bodies in which they live are mostly seawater. After entering rivers and artificial breeding environments, they may be stimulated by the low temperature environment and cause mass death. temperature will cause a huge loss
For puffer obscurus, no related studies and SNP reports of these two genes have been published

Method used

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  • Takifugu obscurus SNP molecular markers and application thereof in genetic breeding
  • Takifugu obscurus SNP molecular markers and application thereof in genetic breeding
  • Takifugu obscurus SNP molecular markers and application thereof in genetic breeding

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Embodiment Construction

[0032] The present invention is based on the two pairs of primers described in SEQ ID NO: 3 and SEQ ID NO: 4 and SEQ ID NO: 5 and SEQ ID NO: 6 for extraction of genomic DNA of Fugu obscurus, PCR amplification, sequencing of amplified products, and Analysis of sequencing results. Obtained the SNP site (SNPg.120C> A) and SNP sites that are significantly related to cold tolerance (SNPg.41A> T). By inputting the relevant biological information of the two sites into the genetic quality judgment system of the present invention, the genetic quality judgment program related to the growth and cold resistance of the obscured puffer can be obtained, which can be applied to the current and future obscured oriental Takifugu molecular markers assist the breeding process.

[0033] The main steps of the process include:

[0034] (a) Obscuration of the obscured puffer population;

[0035] (b) Genomic DNA extraction of Fugu obscurus;

[0036] (c) PCR amplify the genomic DNA of Takifugu obscurus base...

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Abstract

The invention discloses takifugu obscurus SNP molecular markers and application thereof in genetic breeding. The takifugu obscurus SNP molecular markers includes the SNP molecular marker TGF120 and the SNP molecular marker CIRP41, wherein the SNP molecular marker TGF120 is related to weight and standard head length, and the SNP molecular marker CIRP41 is related to cold resistance; amino acid sequences where the SNP molecular markers are located are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively; the TGF120 is located at a 120th site, namely an m with a corresponding base of C or A, of thesequence shown in SEQ ID NO:1; and the CIRP41 is located at a 41th site, namely a y with a corresponding base of A or T, of the sequence shown in SEQ ID NO:2. By adopting the takifugu obscurus SNP molecular markers, takifugu obscurus breeding parents which grow well and resist low temperature can be effectively selected, and thus tetraodontidae variety improving and industry developing are promoted. The invention further provides a takifugu obscurus genetic quality judgement system. Therefore, workloads of breeding selection can be reduced, and the work efficiency is improved.

Description

Technical field [0001] The invention belongs to aquatic breeding technology, and particularly relates to the SNP molecular marker of obscured puffer and its application in genetic breeding. Background technique [0002] Takifugu fasciatus (Takifugu fasciatus), commonly known as pufferfish, is a valuable economic fish of the order Bonefish, Radial fish, and Takifugu. It is widely distributed in coastal areas and inland rivers of China, Japan and the Korean Peninsula. With the development of industrial technology, fishing technology and efficiency have been rapidly improved. Uncontrolled fishing and emission pollution have caused the population of obscured puffer fish to decline rapidly. The fishing flood of wild obscured puffer has basically disappeared, seriously threatening ecological diversity and Stability has also caused a blow to the trade economy of the obscured puffer. Therefore, artificial selection of rare fish species such as obscured puffer fish to alleviate the contr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11G16B40/10G16B50/30G16B20/40
CPCC12Q1/6888G16B40/10G16B50/30G16B20/40C12Q2600/124C12Q2600/156C12Q2600/172Y02A40/81
Inventor 尹绍武张鑫宇裴雪莹张红燕王涛郑翔
Owner NANJING NORMAL UNIVERSITY
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