Takifugu obscurus SNP molecular markers and application thereof in genetic breeding
A technology of puffer obscurus and molecular markers, which is applied in the direction of recombinant DNA technology, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of few edible parts, short trunk, and no release, etc., and achieve The results are accurate and stable, the data capacity is huge, and the conditions and results are clear and intuitive.
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[0032] The present invention is based on the two pairs of primers described in SEQ ID NO: 3 and SEQ ID NO: 4 and SEQ ID NO: 5 and SEQ ID NO: 6 for extraction of genomic DNA of Fugu obscurus, PCR amplification, sequencing of amplified products, and Analysis of sequencing results. Obtained the SNP site (SNPg.120C> A) and SNP sites that are significantly related to cold tolerance (SNPg.41A> T). By inputting the relevant biological information of the two sites into the genetic quality judgment system of the present invention, the genetic quality judgment program related to the growth and cold resistance of the obscured puffer can be obtained, which can be applied to the current and future obscured oriental Takifugu molecular markers assist the breeding process.
[0033] The main steps of the process include:
[0034] (a) Obscuration of the obscured puffer population;
[0035] (b) Genomic DNA extraction of Fugu obscurus;
[0036] (c) PCR amplify the genomic DNA of Takifugu obscurus base...
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