Lactobacillus gasseri lg08 capable of degrading uric acid and its application

A kind of Lactobacillus gasseri, the technology of action, applied in the field of microorganisms, can solve the problems of whether unreported strains can degrade uric acid, affect the mitigation effect, degrade inosinic acid reports and the like

Active Publication Date: 2022-05-27
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it should be pointed out that there are many precursors that can synthesize uric acid contained in food. For example, some umami flavor agents that improve flavor contain not only disodium 5'-guanylate, but also disodium 5'-inosinate. None of the above strains have been reported to degrade inosinic acid
In addition, uric acid is also continuously produced during the body's own metabolic process. Although the above-mentioned strains can efficiently metabolize part of the precursors of uric acid synthesis, it has not been reported whether the above-mentioned strains can degrade the produced uric acid, which directly affects their response to the reduction of uric acid excretion. The relieving effect of hyperuricemia, such as primary hyperuricemia caused by decreased glomerular filtration, increased renal tubular reabsorption, and decreased renal tubular secretion, and this type of hyperuricemia accounted for the primary About 90% of episodic hyperuricemia

Method used

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  • Lactobacillus gasseri lg08 capable of degrading uric acid and its application
  • Lactobacillus gasseri lg08 capable of degrading uric acid and its application
  • Lactobacillus gasseri lg08 capable of degrading uric acid and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of strains with degrading uric acid

[0026] 1) strain screening

[0027] Extract 5 mL of diluent from the feces of healthy elderly people in Bama Town, Hechi City, Guangxi Zhuang Autonomous Region, China (the population has not taken antibiotics before collection, no history of taking probiotics, and no history of gastrointestinal disease), and serially diluted 10 times to 10 -6 , 200 μL of each dilution concentration was spread on selective MRS medium, anaerobic culture at 37°C for 48-72h, and different single colonies on the plate were obtained by separation. Using the sterilized inoculation loop, pick the above-mentioned different single colonies on MRS solid medium for streak purification and anaerobic culture at 37°C for 48h to obtain a total of 9 strains of pure lactic acid bacteria, named L1-L9 respectively.

[0028] 2) Analysis of the tolerance of isolated strains to simulated gastric juice

[0029] After collecting and culturing, the cell...

Embodiment 2

[0046] Example 2 Identification of strains

[0047] According to the bacterial strain L8 obtained in Example 1, the identification method is as follows:

[0048] The obtained strain L8 was identified by 16s rDNA sequencing and physiological and biochemical identification. The 16s rDNA universal primer sequence 27F is: 5'-AGAGTTTGATCCTGGCTCAG-3' (Seq ID No: 1), 1492R is: 5'-GGTTACCTTGTTACGACTT-3' (Seq ID No: 2).

[0049] The 16S rDNA gene sequence of strain L8 was amplified and sequenced, and the PCR amplification product was sent to Shanghai Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing. The nucleotide sequence of the 16S rDNA of strain L8 is shown in Seq ID No: 3 . After 16s rDNA gene comparison, the similarity rate with the Lactobacillus gasseri strain in Genebank reached 100%. Combined with the results of physiological and biochemical identification, strain L8 was identified as a Lactobacillus gasseri strain and named Lactobacillus gasseri LG08. Its physiologi...

Embodiment 3

[0054] Example 3 Analysis of Lactobacillus gasseri LG08 degraded nucleosides and nucleotide products

[0055] This embodiment provides a method for detecting the metabolites after Lactobacillus gasseri LG08 degrades adenosine, guanosine, inosine, adenylic acid, guanylic acid, inosinic acid and disodium 5'-guanylate , which includes the following steps:

[0056] Add 5 mmol / L adenosine, guanosine, inosine, adenylic acid, guanylic acid, inosinic acid and disodium 5'-guanylate to the ammonium acetate buffer solution, and the concentration is 0.4g according to the dry weight of the bacteria. After washing the cells with PBS buffer / L, the cells were mixed and cultured at 37°C for 2 hours and then disrupted by ultrasonic wave. The crushing conditions were as follows: put the suspension into an ice-water bath and adopt a strategy of over 3s and 5s (250w), then 4°C, 12000rpm After centrifugation for 15 min, the supernatant was collected and the concentrations of metabolites (hypoxanth...

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Abstract

The invention relates to the field of microorganisms, in particular to a Lactobacillus gasseri LG08 capable of degrading uric acid and an application thereof. The Lactobacillus gasseri LG08 of the present invention has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on July 18, 2018, the preservation number is CGMCC No.16131, and the classification is named Lactobacillus gasseri (Lactobacillus gasseri), The preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 1, Beichen West Road, Chaoyang District, Beijing. It can also directly and efficiently decompose uric acid in the intestinal tract, and further inhibit the production of uric acid and relieve inflammation and other side effects by reducing the level of serum endotoxin and inhibiting the activity of xanthine oxidase.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to Lactobacillus gasseri LG08 with uric acid degrading effect and application thereof. Background technique [0002] In recent years, with the continuous improvement of people's material living standards, the public's intake of high-purine foods such as seafood, meat and beer has continued to increase, which not only improves the happiness of the tongue, but also brings many chronic metabolic diseases. , such as hyperuricemia. According to statistics, there are about 120 million patients with hyperuricemia in my country, which is close to 10% of the total population. Among them, middle-aged and elderly men and postmenopausal women are the high-risk groups. Under normal circumstances, uric acid in the human body is continuously generated and excreted, and maintained within a certain concentration range. However, once this balance is disturbed, it is easy to cause an increase in the le...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P19/06C12R1/225
CPCA61K35/747A61P19/06C12R2001/225C12N1/205
Inventor 方曙光吴明科朱建国刘春
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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