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Application of Quinazolinone Alkaloid Compound Derived from Marine Fungi in Control of Marine Fouling Organisms

A technology of quinazolinone and marine fungus, applied in the field of application of quinazolinone alkaloid compounds in marine fouling biological control, achieving good application prospects, great potential for promotion and application, and good social benefits

Active Publication Date: 2020-01-24
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, in the field of marine antifouling, there is no relevant report disclosing the role of such compounds in biofouling control

Method used

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  • Application of Quinazolinone Alkaloid Compound Derived from Marine Fungi in Control of Marine Fouling Organisms
  • Application of Quinazolinone Alkaloid Compound Derived from Marine Fungi in Control of Marine Fouling Organisms

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Experimental program
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Effect test

Embodiment 1

[0028] The quinazolinone alkaloid compound of the present invention can be isolated from the cell of the marine fungus Aspergillus sp. SK-28. The marine fungus was isolated from the leaves of the mangrove plant Candela in the Shankou Mangrove Reserve of Guangxi Zhuang Autonomous Region; the specific steps are as follows:

[0029] 1. Seed culture:

[0030] 1.1 Preparation of seed medium: 200g of potatoes, 20g of glucose, 1L of tap water, equally distributed in five 500mL Erlenmeyer flasks, and extinguished at 121°C for 30 minutes.

[0031] 1.2 Cultivation of seeds: Inoculate the strains of marine fungi into the seed medium, place them on a shaker at a speed of 200 rpm at a temperature of 28° C., and cultivate them for 72 hours to obtain a seed culture solution.

[0032] 2. Fermentation culture:

[0033] 2.1 Prepare fermentation medium: 6000g rice, 180g sea salt, 6L tap water, extinguish at 121°C for 30 minutes.

[0034] 2.2 Fermentation culture: Aseptically transfer 5 mL of ...

Embodiment 2

[0038] Anti-biofouling activity experiment is carried out to the quinazolinone alkaloid compound in embodiment 1:

[0039] In the experimental group, the quinazolinone alkaloid compound was dissolved in methanol to prepare a solution with a concentration of 282.6 μg / mL. Add 1 mL of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the quinazolinone alkaloid compound coated on the bottom of the petri dish is 10 μg / cm 2 .

[0040]Add 1mL of methanol to the control group, so that the solution is evenly distributed on the bottom of the petri dish, and then add 10mL of seawater after the solvent is completely evaporated.

[0041] Only 10 mL of seawater was added to the blank group.

[0042] Three parallel samples were set up for each of the experimental group, the control group and the blank group. Add 30 netted barnacle Venus larvae to each sample. Place them...

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Abstract

The invention discloses an application of a quinazolinone alkaloid compound derived from marine fungi in the prevention and control of marine fouling organisms. The application of the quinazolinone alkaloid compound in the prevention and control of marine fouling organisms is disclosed for the first time, and the quinazolinone alkaloid compound has a significant inhibitory effect on the adhesion of marine fouling organisms on solids, and has a significant effect under low content coating. At the same time, when inhibiting the attachment of marine organisms, it will not kill marine organisms, but will play a repellent role. The compound can not only be extracted from the fermentation products of marine fungi, but also be obtained through artificial mature synthesis process, has reliable andstable sources, and has good application prospects in the prevention and control of fouling organisms in various marine artificial facilities.

Description

technical field [0001] The invention belongs to the field of marine antifouling coatings, and in particular relates to the application of a marine fungus-derived quinazolinone alkaloid compound in marine fouling biological control. Background technique [0002] Marine biofouling refers to the phenomenon that various artificial facilities or ships soaked in seawater are attached to marine microorganisms, plants or animals and have adverse effects on human economic activities. The main harm is to increase the roughness and weight of ships, thereby increasing Large resistance, reducing speed, increasing fuel consumption and hull maintenance costs; hindering the normal work of various marine facilities, causing drift, imbalance and even capsizing; in marine aquaculture, it will also compete with cultured shellfish for attachment base and bait, Or blockage of cages leads to poor water flow and insufficient oxygen supply, which affects the output and quality of aquaculture aquatic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09D5/16C12P17/18C12R1/66
CPCC09D5/1625C12P17/182
Inventor 佘志刚严涛蔡润林曹文浩
Owner SUN YAT SEN UNIV
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