Application of a marine fungus-derived beauveria in biological control of marine fouling
A beauvericin, marine fouling technology, used in antifouling/underwater coatings, biocide-containing paints, coatings, etc.
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Embodiment 1
[0021] The beauveria of the present invention can be isolated from the thallus of mangrove-derived fungi. Specific steps are as follows:
[0022] Use rice medium to ferment and cultivate the endophytic fungi of mangrove forests, and culture them statically for 28 days. The fermented product is soaked in ethyl acetate. The crude extract was separated by silica gel column chromatography, and the beauveriacin compound represented by the above formula was obtained in the isocratic eluent of petroleum ether / ethyl acetate (volume ratio: 7 / 3).
Embodiment 2
[0024] Anti-biofouling activity experiment is carried out to the beauveria in embodiment 1:
[0025] In the experimental group, the beauverum compound was dissolved in methanol to prepare a solution with a concentration of 282.6 μg / mL. Add 1 mL of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely volatilized, the content of beauvericin coated on the bottom of the petri dish is 10 μg / cm 2 .
[0026] Add 1mL of methanol to the control group, so that the solution is evenly distributed on the bottom of the petri dish, and then add 10mL of seawater after the solvent is completely evaporated.
[0027] Only 10 mL of seawater was added to the blank group.
[0028] Three parallel samples were set up for each of the experimental group, the control group and the blank group. Add 30 netted barnacle Venus larvae to each sample. Place them in a constant temperature incubator at a temperature of ...
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